School of Environment, Nanjing Normal University, Nanjing 210023, China.
State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Surficial Geochemistry, School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China.
Sci Total Environ. 2020 Jul 1;724:138257. doi: 10.1016/j.scitotenv.2020.138257. Epub 2020 Mar 27.
A mechanistic understanding of perfluorooctanoic acid (PFOA) toxicity to plants is essential for future risk assessment of PFOA in agricultural soil. In this study, soil-grown cucumber (Cucumis sativus) was exposed to 0, 0.2, and 5 mg/kg of PFOA for 60 days. At harvest, contaminant accumulation, cucumber biomass, photosynthesis profiles and metabolites were measured. Results showed that PFOA depressed cucumber biomass and accumulated highest in leaves. Photosynthesis analysis revealed that PFOA at both doses reduced the chlorophyll contents and net photosynthesis rate of cucumber leaves. Gas chromatography-mass spectrometry-based non-targeted metabolomics revealed that PFOA induced metabolic reprogramming in cucumber leaves, including up-regulation of phenols (at 0.2 and 5 mg/kg) and down-regulation of amino acids (at 5 mg/kg), indicating disrupted nitrogen and carbon metabolism. Results revealed how PFOA represses plant growth by down-regulating photosynthetic pigments and disturbing the metabolism of carbohydroxides, phenols and amino acids. These findings provide valuable information for understanding the molecular mechanisms involved in plant responses to PFOA-induced stress.
了解全氟辛酸（PFOA）对植物的毒性的机理对于未来评估农业土壤中 PFOA 的风险至关重要。在这项研究中，用 0、0.2 和 5mg/kg 的 PFOA 处理生长在土壤中的黄瓜（Cucumis sativus）60 天。收获时，测量污染物积累、黄瓜生物量、光合作用谱和代谢物。结果表明，PFOA 抑制了黄瓜生物量的生长，在叶片中积累最多。光合作用分析表明，两种剂量的 PFOA 均降低了黄瓜叶片的叶绿素含量和净光合速率。基于气相色谱-质谱的非靶向代谢组学揭示，PFOA 诱导了黄瓜叶片的代谢重编程，包括酚类物质（在 0.2 和 5mg/kg 时上调）和氨基酸（在 5mg/kg 时下调），表明氮和碳代谢受到干扰。结果表明，PFOA 通过下调光合色素和扰乱碳水化合物、酚类和氨基酸的代谢来抑制植物生长。这些发现为理解植物对 PFOA 诱导的应激反应的分子机制提供了有价值的信息。
