School of Life Science and Engineering, Lanzhou University of Technology, Gansu Province Key Laboratory of Complementary Energy System of Biomass and Solar Energy, 287 Langongping Road, Lanzhou, Gansu Province 730050, PR China.
Institute of Biology, Gansu Academy of Sciences, 229 South Dingxi Road, Lanzhou, Gansu Province 73000, PR China.
Bioresour Technol. 2020 Jul;307:123238. doi: 10.1016/j.biortech.2020.123238. Epub 2020 Mar 23.
In order to enable rapid disposal and proper preservation of discarded vegetable for waste valorization, ensiling was employed to preserve cauliflower leaves for 30 days at different temperatures (20 ~ 45 °C) with and without the addition of Lactobacillus plantarum L8. The L. plantarum inoculant reduced dry matter (DM) loss and enhanced the preservation of protein and soluble carbohydrate while decreasing pH and ammonia nitrogen content. The silages at 35 °C exhibited the best fermentation profile characterized by the highest lactic acid content (185 g·kg DM) and the lowest pH (4.08) and ammonia nitrogen content (37.6 g·kg total nitrogen) with L. plantarum inoculation. The presence of exogenous L. plantarum improved the silage fermentation, enriched Lactobacillus and Weissella, and reduced the microbial richness/diversity, resulting in efficient lactic acid fermentation, especially at 30 and 35 °C. Moreover, the microbial community dynamics was correlated with the chemical compositions and fermentation metabolites in silages.
为了实现废弃蔬菜的快速处理和妥善保存,以实现废物增值,本研究采用青贮的方法,在不同温度(20~45°C)下,添加和不添加植物乳杆菌 L8 对菜花叶进行了 30 天的保存。植物乳杆菌接种剂减少了干物质(DM)损失,提高了蛋白质和可溶性碳水化合物的保存效果,同时降低了 pH 值和氨态氮含量。在 35°C 下进行青贮时,发酵特性最佳,具有最高的乳酸含量(185 g·kg DM)、最低的 pH 值(4.08)和氨态氮含量(37.6 g·kg 总氮),且添加了植物乳杆菌。外源植物乳杆菌的存在改善了青贮发酵,富集了乳杆菌属和魏斯氏菌属,降低了微生物丰富度/多样性,导致乳酸发酵效率提高,特别是在 30 和 35°C 时。此外,微生物群落动态与青贮饲料的化学成分和发酵代谢物密切相关。