Huang Rongzheng, Chai Yuxin, Li Shuangming, Chen Yongcheng, Jia Shu'an, Ma Chunhui, Zhang Fanfan
Grassland Science, School of Animal Technology, Shihezi University, Shihezi, Xinjiang, China.
Microbiol Spectr. 2025 Jun 3;13(6):e0266724. doi: 10.1128/spectrum.02667-24. Epub 2025 Apr 30.
Nettle showed several benefits for animals as an unconventional feed source, but it is difficult to ensile in order to be better utilized. Recently, nettle has been utilized to alternate alfalfa as ruminant feed, but it is usually poor in ensiling quality. To determine the endogenous component influencing ensiling, we investigated the effect of on the characteristics of nettle silage and the mechanism of action of these substances against . Inoculation with decreased the relative abundance of by 85.95% in the middle fermentation stage of nettle silage (30 d), with a 24.74% decrease in the ammonia content (＜0.05). In the correlation analysis, 4-pentenoic acid showed an extremely significant negative correlation with spp. in nettle silage ( < 0.001). After 4-pentenoic acid treatment, the most downregulated proteins were involved in the ribosome pathway (30 differentially expressed proteins), and then in the glycolysis/gluconeogenesis, pyruvate metabolism, and fatty acid synthesis pathways (particularly for A, D, and G). The mechanism of action of 4-pentenoic acid against mainly involves inhibition of fatty acid synthesis and decreased the expression of acid tolerance proteins. The present study will give new insights into silage fermentation and provide new clues for better ensiling of nettle.
Nettle has attracted the attention of scientists due to its several benefits for animals as non-conventional feed sources. However, as for challenge, nettle is difficult to ensile (poor quality), which is an obstacle for nettle use. In the present manuscript, we investigated the effect of on the characteristics of nettle silage and clarified the mechanisms of 4-pentenoic acid against . Our findings suggested that could improve nettle silage quality at a significant level through decreased production of ammonia (decline percentage was 21.41%-31.73%) during ensiling, while it could not well improve the quality of nettle silage due to the interference effect of 4-pentenoic acid as an antibacterial substance. The mechanism of 4-pentenoic acid against was mainly through inhibition of fatty acid synthesis (fabG) and expression of acid tolerance protein (accA), resulting in destruction of the cell wall in Our finding could give a new clue for better use of nettle silage.
荨麻作为一种非常规饲料来源对动物有多种益处，但为了更好地利用它，青贮却很困难。最近，荨麻已被用作反刍动物饲料来替代苜蓿，但它的青贮质量通常较差。为了确定影响青贮的内源性成分，我们研究了[具体物质]对荨麻青贮特性的影响以及这些物质对抗[具体微生物]的作用机制。在荨麻青贮的中期发酵阶段（30天），接种[具体物质]使[具体微生物]的相对丰度降低了85.95%，氨含量降低了24.74%（<0.05）。在相关性分析中，4-戊烯酸与荨麻青贮中的[具体微生物]呈现极显著负相关（<0.001）。经4-戊烯酸处理后，下调最明显的蛋白质参与核糖体途径（30个差异表达蛋白），其次是糖酵解/糖异生、丙酮酸代谢和脂肪酸合成途径（特别是A、D和G）。4-戊烯酸对抗[具体微生物]的作用机制主要涉及抑制脂肪酸合成并降低耐酸蛋白的表达。本研究将为青贮发酵提供新的见解，并为荨麻更好地青贮提供新线索。
荨麻因其作为非常规饲料来源对动物有多种益处而受到科学家的关注。然而面临的挑战是，荨麻难以青贮（质量差），这是荨麻利用的一个障碍。在本论文中，我们研究了[具体物质]对荨麻青贮特性的影响，并阐明了4-戊烯酸对抗[具体微生物]的机制。我们的研究结果表明，[具体物质]可通过在青贮过程中减少氨的产生（下降百分比为21.41%-31.73%）显著提高荨麻青贮质量，而由于4-戊烯酸作为抗菌物质的干扰作用，它不能很好地提高荨麻青贮质量。4-戊烯酸对抗[具体微生物]的机制主要是通过抑制脂肪酸合成（fabG）和耐酸蛋白（accA）的表达，导致[具体微生物]细胞壁被破坏。我们的研究结果可为更好地利用荨麻青贮提供新线索。
