Miyake M, Fuchimoto S, Orita K
Department of Surgery, University of Okayama, Japan.
Exp Cell Biol. 1988;56(6):297-302. doi: 10.1159/000163495.
We tested the antiproliferative effect induced by the natural human tumor necrosis factors alpha and beta (nHuTNF-alpha, -beta) or a combination of these in the clonogenic assay. The antiproliferative effects were evaluated by examining the inhibition of clonogenic growth of RPMI-4788 cells, which had been established from a human colon cancer. TNF-alpha and -beta were natural human types produced by a B cell leukemia line (BALL-1 cells) and were both over 99% pure. The antiproliferative effect in combination of nHuTNF-alpha and -beta was analysed by using the median effect plot and the combination index. The results indicate a synergism between two factors.
我们在克隆形成试验中测试了天然人肿瘤坏死因子α和β（nHuTNF-α、-β）或二者组合诱导的抗增殖作用。通过检测源自人结肠癌的RPMI-4788细胞克隆形成生长的抑制情况来评估抗增殖作用。TNF-α和-β是由B细胞白血病系（BALL-1细胞）产生的天然人类型，纯度均超过99%。采用中位效应图和组合指数分析nHuTNF-α和-β联合使用时的抗增殖作用。结果表明这两种因子之间存在协同作用。
