Zhao Mengge, Ma Aimin, He Hui, Guo Danjun, Hou Tao
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan 43000, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan 43000, China.
Food Res Int. 2020 May;131:108974. doi: 10.1016/j.foodres.2019.108974. Epub 2020 Jan 21.
Two novel calcium delivery systems with high calcium-binding ability were prepared with desalted duck egg white peptides (DPs) and chitosan oligosaccharide (COS) by Amadori-type linkage and transglutaminase (TGase) induced reaction. Fourier transform infrared spectroscopy (FTIR), Zeta potential and particle size analyses were used to characterize the copolymers. These two copolymers reversed the inhibition of phytic acid (PA) in calcium transport in Caco-2 cell monolayers. PA-induced calcium deficiency mice model indicated that copolymers could reverse the side effect of PA, promote calcium bioavailability, and improve gut health. Overall, these results confirmed the potentialities of DPs-COS copolymers as nutraceuticals/functional food for oral calcium delivery to promote calcium absorption and improve gut health.
通过阿玛多利型键合和转谷氨酰胺酶（TGase）诱导反应，用脱盐鸭蛋清肽（DPs）和壳寡糖（COS）制备了两种具有高钙结合能力的新型钙递送系统。采用傅里叶变换红外光谱（FTIR）、Zeta电位和粒度分析对共聚物进行表征。这两种共聚物逆转了植酸（PA）对Caco-2细胞单层钙转运的抑制作用。PA诱导的缺钙小鼠模型表明，共聚物可以逆转PA的副作用，提高钙的生物利用度，并改善肠道健康。总体而言，这些结果证实了DPs-COS共聚物作为营养保健品/功能性食品用于口服钙递送以促进钙吸收和改善肠道健康的潜力。
