Hou Tao, Wang Chi, Ma Zhili, Shi Wen, Weiwei Lui, He Hui
College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.
Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
J Agric Food Chem. 2015 Sep 23;63(37):8170-6. doi: 10.1021/acs.jafc.5b03097. Epub 2015 Sep 10.
The effects of desalted duck egg white peptides (DPs) on calcium absorption were investigated in three models: Caco-2 cell monolayer model, Caco-2 cell population model, and everted intestinal sac model. DPs were found to enhance calcium transport and may do so by acting as calcium carriers and interacting with the cell membrane to open a special Ca(2+) channel, whereas the paracellular pathway may make only a minor contribution. Structure characterization demonstrated the important roles of seven crucial peptides, such as VSEE and LYAEE, in binding calcium and promoting calcium uptake. Three synthetic peptides (VHSS, VSEE, and VHS(p)S(p)) potently induced calcium transport in Caco-2 monolayers, with VHS(p)S(p) being the most effective. This research expands the understanding of the mechanism of cellular calcium uptake by DPs as well as highlights an opportunity for recycling an otherwise discarded processing byproduct.
在三种模型中研究了脱盐鸭蛋清肽（DPs）对钙吸收的影响：Caco-2细胞单层模型、Caco-2细胞群体模型和外翻肠囊模型。发现DPs可增强钙转运，其作用方式可能是作为钙载体并与细胞膜相互作用以打开特殊的Ca(2+)通道，而细胞旁途径的贡献可能较小。结构表征表明七种关键肽（如VSEE和LYAEE）在结合钙和促进钙摄取方面具有重要作用。三种合成肽（VHSS、VSEE和VHS(p)S(p)）能有效诱导Caco-2单层细胞中的钙转运，其中VHS(p)S(p)最为有效。本研究扩展了对DPs细胞钙摄取机制的理解，同时也凸显了回收原本被丢弃的加工副产品的机会。
