College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, PR China.
College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, PR China.
Bioresour Technol. 2020 Jul;308:122935. doi: 10.1016/j.biortech.2020.122935. Epub 2020 Jan 31.
Accelerated denitrification is an essential problem in the biological treatment of nitrogenous wastewater. In this study, we report that denitrification is accelerated by micro-graphite particles (MGPs). The denitrification rate was increased by 83.4% or 11.1% in synthetic (with 0.16 g/L MGPs) or industrial nitrogenous wastewater (with 0.12 g/L MGP), respectively. The mechanism was revealed via a quantitative polymerase chain reaction (q-PCR), high-throughput sequencing, and scanning electron microscopy (SEM). The abundance of denitrifying bacteria Paracoccus in the sludge was increased by micro-graphite particles. The number of denitrifying bacteria with the nirS gene was increased significantly (75.6%). To the best of our knowledge, this is the first report that MGP could enhance denitrification via the sludge. MGP can denitrify in industrial applications.
加速脱氮是生物处理含氮废水的一个重要问题。在本研究中，我们报告称，微石墨颗粒（MGPs）可加速脱氮。在合成废水（含 0.16 g/L MGPs）或工业含氮废水中，脱氮速率分别提高了 83.4%或 11.1%。通过定量聚合酶链反应（q-PCR）、高通量测序和扫描电子显微镜（SEM）揭示了这一机制。微石墨颗粒使污泥中反硝化菌 Paracoccus 的丰度增加。带有nirS 基因的反硝化菌数量显著增加（75.6%）。据我们所知，这是第一个报道 MGPs 可以通过污泥来增强脱氮的研究。MGPs 可以在工业应用中进行反硝化。
