Rauzier J, Moniz-Pereira J, Gicquel-Sanzey B
Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
Gene. 1988 Nov 30;71(2):315-21. doi: 10.1016/0378-1119(88)90048-0.
We have determined the complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum; the plasmid contains 4837 bp with 65% G + C. Five open reading frames (ORF1 to ORF5) have been identified. A number of sequences corresponding to palindromes, repeats, a helix-turn-helix motif, a signal sequence and repetitive amino acid motifs can be identified. This sequence should facilitate the construction of vectors based on pAL5000 for transfer and expression studies in mycobacteria.
我们已经确定了来自偶然分枝杆菌的质粒pAL5000的完整核苷酸序列；该质粒含有4837个碱基对，G + C含量为65%。已鉴定出五个开放阅读框（ORF1至ORF5）。可以识别出许多与回文序列、重复序列、螺旋-转角-螺旋基序、信号序列和重复氨基酸基序相对应的序列。该序列应有助于构建基于pAL5000的载体，用于分枝杆菌的转移和表达研究。
