Rossi M, Brigidi P, Gonzalez Vara y Rodriguez A, Matteuzzi D
Department of Pharmaceutical Sciences, University of Bologna, Italy.
Res Microbiol. 1996 Mar-Apr;147(3):133-43. doi: 10.1016/0923-2508(96)80213-0.
The nucleotide sequence of the 1847-bp Bifidobacterium longum B2577 cryptic plasmid pMB1 was determined. The plasmid had a G+C content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon. The proteins encoded by orf1 and orf2 show the highest degree of similarity with similarly arranged peptide sequences translated from Corynebacterium glutamicum pXZ10142 and Mycobacterium fortuitum pAL5000 plasmids. Recombinant plasmids containing the pMB1 replicon were able to replicate in Bifidobacterium animalis MB209. The successful transformation of this strain with pMB1-based plasmids facilitated characterization of this replicon, results of which showed that both orf1 and orf2 are necessary for plasmid replication. A family of new Escherichia coli-B. animalis shuttle plasmids, based on the pMB1 replicon and expressing a cat and an ery gene, was constructed.
测定了长双歧杆菌B2577隐蔽质粒pMB1的1847 bp核苷酸序列。该质粒的G+C含量为62.0%，包含两个开放阅读框orf1和orf2，可能以操纵子形式排列。由orf1和orf2编码的蛋白质与从谷氨酸棒杆菌pXZ10142和偶然分枝杆菌pAL5000质粒翻译的类似排列的肽序列具有最高程度的相似性。含有pMB1复制子的重组质粒能够在动物双歧杆菌MB209中复制。用基于pMB1的质粒成功转化该菌株有助于对该复制子进行表征，结果表明orf1和orf2对于质粒复制都是必需的。构建了一个基于pMB1复制子、表达cat和ery基因的新型大肠杆菌-动物双歧杆菌穿梭质粒家族。
