Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei, China.
Plant Signal Behav. 2020 May 3;15(5):1748282. doi: 10.1080/15592324.2020.1748282. Epub 2020 Apr 5.
As an apoplast signal molecule, extracellular ATP (eATP) is involved in the growth regulation of Arabidopsis thaliana seedlings. Recently, RRFT1 was revealed to be involved in eATP- regulated seedling growth. To further verify the role of RRTF1 in seedlings' eATP response, expression of 20 eATP-responsive genes in wild type (Col-0) and RRTF1 null mutant (rrtf1-1) seedlings were investigated by using realtime quantitative PCR. After 0.5 mM ATP stimulation, the response of these genes' expression in rrtf1-1 seedlings was significantly different from that in Col-0 seedlings. Proteins which are encoded by these genes include transcription factors, plasma membrane receptors like kinases, ion influx/efflux transporters and hormone signaling components. The results indicated that RRTF1 may be involved in eATP regulated physiological responses via regulating the expression of some functional genes.
作为一种质外体信号分子，细胞外三磷酸腺苷（eATP）参与拟南芥幼苗的生长调控。最近，RRFT1 被揭示参与 eATP 调节的幼苗生长。为了进一步验证 RRTF1 在幼苗 eATP 反应中的作用，通过实时定量 PCR 研究了野生型（Col-0）和 RRTF1 缺失突变体（rrtf1-1）幼苗中 20 个 eATP 响应基因的表达。在 0.5 mM ATP 刺激后，rrtf1-1 幼苗中这些基因表达的响应与 Col-0 幼苗显著不同。这些基因编码的蛋白质包括转录因子、类激酶的质膜受体、离子流入/流出转运蛋白和激素信号成分。结果表明，RRTF1 可能通过调节一些功能基因的表达参与 eATP 调节的生理反应。
