Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing, China.
The University of Dundee, Division of Plant Sciences at the James Hutton Institute, DD2 5DA, U.K.
Phytopathology. 2020 Aug;110(8):1375-1387. doi: 10.1094/PHYTO-09-19-0331-R. Epub 2020 Jun 16.
Late blight is a devastating potato disease worldwide, caused by . The strain 2013-18-306 from Yunnan is a "supervirulent race" that overcomes all 11 known late blight resistance genes ( to ) from . In a previous study, we identified a diploid wild-type potato JAM1-4 () with high resistance to 2013-18-306. dRenSeq analysis indicated the presence of novel genes in JAM1-4. RNA-Seq was used to analyze the late blight resistance response genes and defense regulatory mechanisms of JAM1-4 against 2013-18-306. Gene ontology enrichment and KEGG pathway analysis showed that many disease-resistant pathways were significantly enriched. Analysis of differentially expressed genes (DEGs) revealed an active disease resistance mechanism of JAM1-4, and the essential role of multiple signal transduction pathways and secondary metabolic pathways comprised of SA-JA-ET in plant immunity. We also found that photosynthesis in JAM1-4 was inhibited to promote the immune response. Our study reveals the pattern of resistance-related gene expression in response to a super race strain of potato late blight and provides a theoretical basis for further exploration of potato disease resistance mechanisms, discovery of new late blight resistance genes, and disease resistance breeding.
晚疫病是一种全球性的毁灭性马铃薯病害，由 引起。来自云南的 2013-18-306 菌株是一种“超级毒力菌株”，克服了来自 的所有 11 个已知晚疫病抗性基因（ ）。在之前的研究中，我们鉴定了一个对 2013-18-306 具有高抗性的二倍体野生型马铃薯 JAM1-4（ ）。dRenSeq 分析表明 JAM1-4 中存在新型 基因。使用 RNA-Seq 分析 JAM1-4 对 2013-18-306 的晚疫病抗性反应基因和防御调控机制。基因本体富集和 KEGG 通路分析表明，许多抗病途径显著富集。差异表达基因（DEGs）分析表明 JAM1-4 具有活跃的抗病机制，多条信号转导途径和由 SA-JA-ET 组成的次级代谢途径在植物免疫中起着重要作用。我们还发现 JAM1-4 中的光合作用受到抑制，以促进免疫反应。我们的研究揭示了马铃薯晚疫病超级毒力菌株诱导的抗性相关基因表达模式，为进一步探索马铃薯抗病机制、发现新的晚疫病抗性基因和抗病性育种提供了理论依据。
