Chair of Crop Science and Plant Biology, Estonian University of Life Sciences, Kreutzwaldi 1, Tartu 51006, Estonia.
Chair of Crop Science and Plant Biology, Estonian University of Life Sciences, Kreutzwaldi 1, Tartu 51006, Estonia.
Genomics. 2023 Sep;115(5):110678. doi: 10.1016/j.ygeno.2023.110678. Epub 2023 Jul 3.
The Estonia potato cultivar Ando has shown elevated field resistance to Phytophthora infestans, even after being widely grown for over 40 years. A comprehensive transcriptional analysis was performed using RNA-seq from plant leaf tissues to gain insight into the mechanisms activated for the defense after infection. Pathogen infection in Ando resulted in about 5927 differentially expressed genes (DEGs) compared to 1161 DEGs in the susceptible cultivar Arielle. The expression levels of genes related to plant disease resistance such as serine/threonine kinase activity, signal transduction, plant-pathogen interaction, endocytosis, autophagy, mitogen-activated protein kinase (MAPK), and others were significantly enriched in the upregulated DEGs in Ando, whereas in the susceptible cultivar, only the pathway related to phenylpropanoid biosynthesis was enriched in the upregulated DEGs. However, in response to infection, photosynthesis was deregulated in Ando. Multi-signaling pathways of the salicylic-jasmonic-ethylene biosynthesis pathway were also activated in response to Phytophthora infestans infection.
爱沙尼亚土豆品种安德（Ando）在广泛种植 40 多年后，对晚疫病菌表现出了较高的田间抗性。利用 RNA-seq 对植物叶片组织进行了全面的转录组分析，以深入了解感染后激活的防御机制。安德（Ando）受到病原体感染后，与易感品种阿里尔（Arielle）相比，差异表达基因（DEGs）约为 5927 个，而阿里尔（Arielle）中差异表达基因（DEGs）为 1161 个。在安德（Ando）中，与植物抗病性相关的基因，如丝氨酸/苏氨酸激酶活性、信号转导、植物-病原体相互作用、内吞作用、自噬、丝裂原激活蛋白激酶（MAPK）等基因的表达水平显著上调，而在易感品种中，只有苯丙烷生物合成途径与上调的 DEGs 相关。然而，安德（Ando）中，感染后光合作用被失调。水杨酸-茉莉酸-乙烯生物合成途径的多信号通路也被激活，以响应晚疫病菌的感染。
