Bee-DiGregorio Madeleine Y, Feng Hui, Pan Bruce S, Dokoozlian Nick K, Sacks Gavin L
Department of Food Science, Cornell University, 411 Tower Rd, Ithaca, NY 14853, USA.
E&J Gallo Winery, 600 Yosemite Blvd, Modesto, CA 95354, USA.
Foods. 2020 Apr 2;9(4):409. doi: 10.3390/foods9040409.
Etched polymeric sorbent sheets (solid-phase mesh-enhanced sorption from headspace (SPMESH) sheets) were recently described as an alternative to solid-phase microextraction (SPME) for rapid, parallel, multi-sample extraction and pre-concentration of headspace volatiles. In this report, a workflow was evaluated based on SPMESH sheet extraction followed by direct analysis in real time-mass spectrometry (DART-MS) using grape samples harvested from multiple commercial vineyards at different maturities. SPMESH sheet-DART-MS(-MS) was performed on two grape-derived odorants related to wine quality: 3-isobutyl-2-methoxypyrazine (IBMP) in Cabernet Sauvignon and Merlot grape homogenate ( = 86 samples) and linalool in Muscat-type grape juice samples ( = 18 samples). As part of the optimization process, an MS-MS method was developed for IBMP and an equilibration procedure prior to extraction was established for homogenate samples. Following optimization, we achieved good correlation between SPMESH sheet-DART-MS and SPME-GC-MS for both IBMP (range by GC-MS = < 2 ng/L to 28 ng/L, = 0.70) and linalool (range by GC-MS = 135 to 415 μg/L, = 0.66). The results indicate SPMESH sheet-DART-MS is suitable for rapid measurements of trace level volatiles in grapes.
蚀刻聚合物吸附剂片材（顶空固相网增强吸附（SPMESH）片材）最近被描述为一种替代固相微萃取（SPME）的方法，用于快速、并行、多样品萃取和顶空挥发物的预浓缩。在本报告中，评估了一种工作流程，该流程基于SPMESH片材萃取，然后使用从多个商业葡萄园收获的不同成熟度的葡萄样品进行实时质谱直接分析（DART-MS）。对与葡萄酒质量相关的两种葡萄衍生气味物质进行了SPMESH片材-DART-MS（-MS）分析：赤霞珠和梅洛葡萄匀浆（n = 86个样品）中的3-异丁基-2-甲氧基吡嗪（IBMP）以及麝香葡萄汁样品（n = 18个样品）中的芳樟醇。作为优化过程的一部分，开发了一种针对IBMP的质谱-质谱方法，并为匀浆样品建立了萃取前的平衡程序。优化后，对于IBMP（气相色谱-质谱法测定范围 = < 2 ng/L至28 ng/L，r = 0.70）和芳樟醇（气相色谱-质谱法测定范围 = 135至415 μg/L，r = 0.66），我们实现了SPMESH片材-DART-MS与SPME-气相色谱-质谱之间的良好相关性。结果表明，SPMESH片材-DART-MS适用于快速测量葡萄中的痕量挥发性物质。
