描述并分析安哥拉兔（Oryctolagus cuniculus）皮肤和毛囊发育过程中 SIAH1 的功能。

Characterization and functional analysis of SIAH1 during skin and hair follicle development in the angora rabbit (Oryctolagus cuniculus).

机构信息

College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, China.

出版信息

Hereditas. 2020 Apr 6;157(1):10. doi: 10.1186/s41065-020-00126-0.

DOI:10.1186/s41065-020-00126-0

PMID:32252830

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7137485/

Abstract

BACKGROUND

Seven in absentia homolog 1 (SIAH1) is an E3 ubiquitin ligase containing a RING-finger domain and a key regulator of normal development. Skin and hair follicle development is a complex and special process of morphogenesis involving multiple signaling pathways. SIAH1 is enriched in the Wnt signaling pathway and potentially related to hair follicle cycle and skin development. This study aims to provide evidence for the role of SIAH1 in skin and hair development.

RESULTS

Full-length cloning and analysis of SIAH1 was conducted to better understand its function. Phylogenetically, the sequence of SIAH1 in the rabbit shares the greatest homology with Home sapiens, Pongo abelii and Mus mulatta. Based on the rabbit hair follicle synchronization model, we found that the expression level of SIAH1 in the regressive period of the rabbit hair cycle is significantly lower than in the active growth and rest periods. In addition, the mRNA expression levels of skin and hair follicle development-related genes changed significantly when SIAH1 was overexpressed and silenced. After SIAH1 overexpression, the expression levels of WNT2, LEF1 and FGF2 decreased, and those of SFRP2 and DKK1 increased (P < 0.05). After interference of SIAH1, the expression levels of WNT2, LEF1 and FGF2 increased (P < 0.05), and SFRP2 and DKK1 decreased.

CONCLUSIONS

SIAH1 can affect skin and hair follicle development and exert an inhibitory effect. These results could provide foundamental insights into the role of SIAH1 as a target gene in rabbit skin and hair follicle development.

摘要

背景

七次跨膜结构域蛋白 1（SIAH1）是一种 E3 泛素连接酶，含有一个 RING 指结构域，是正常发育的关键调节因子。皮肤和毛囊的发育是一个复杂而特殊的形态发生过程，涉及多个信号通路。SIAH1 在 Wnt 信号通路中富集，可能与毛囊周期和皮肤发育有关。本研究旨在为 SIAH1 在皮肤和毛发发育中的作用提供证据。

结果

对 SIAH1 进行全长克隆和分析，以更好地了解其功能。从系统进化的角度来看，兔 SIAH1 序列与智人、猩猩和猕猴的同源性最高。基于兔毛囊同步模型，我们发现兔毛周期退行期 SIAH1 的表达水平明显低于活跃生长和静止期。此外，当 SIAH1 过表达和沉默时，皮肤和毛囊发育相关基因的 mRNA 表达水平发生显著变化。SIAH1 过表达后，WNT2、LEF1 和 FGF2 的表达水平降低，SFRP2 和 DKK1 的表达水平升高（P<0.05）。干扰 SIAH1 后，WNT2、LEF1 和 FGF2 的表达水平升高（P<0.05），SFRP2 和 DKK1 的表达水平降低。