Zhao Bohao, Chen Yang, Hu Shuaishuai, Yang Naisu, Wang Manman, Liu Ming, Li Jiali, Xiao Yeyi, Wu Xinsheng
College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, China.
Front Genet. 2019 May 3;10:407. doi: 10.3389/fgene.2019.00407. eCollection 2019.
The hair follicle (HF) cycle is a complicated and dynamic process in mammals, associated with various signaling pathways and gene expression patterns. Non-coding RNAs (ncRNAs) are RNA molecules that are not translated into proteins but are involved in the regulation of various cellular and biological processes. This study explored the relationship between ncRNAs and the HF cycle by developing a synchronization model in Angora rabbits. Transcriptome analysis was performed to investigate ncRNAs and mRNAs associated with the various stages of the HF cycle. One hundred and eleven long non-coding RNAs (lncRNAs), 247 circular RNAs (circRNAs), 97 microRNAs (miRNAs), and 1,168 mRNAs were differentially expressed during the three HF growth stages. Quantitative real-time PCR was used to validate the ncRNA transcriptome analysis results. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses provided information on the possible roles of ncRNAs and mRNAs during the HF cycle. In addition, lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA ceRNA networks were constructed to investigate the underlying relationships between ncRNAs and mRNAs. LNC_002919 and novel_circ_0026326 were found to act as ceRNAs and participated in the regulation of the HF cycle as miR-320-3p sponges. This research comprehensively identified candidate regulatory ncRNAs during the HF cycle by transcriptome analysis, highlighting the possible association between ncRNAs and the regulation of hair growth. This study provides a basis for systematic further research and new insights on the regulation of the HF cycle.
毛囊（HF）周期是哺乳动物中一个复杂且动态的过程，与多种信号通路和基因表达模式相关。非编码RNA（ncRNAs）是不翻译成蛋白质但参与各种细胞和生物学过程调控的RNA分子。本研究通过在安哥拉兔中建立同步模型来探索ncRNAs与HF周期之间的关系。进行转录组分析以研究与HF周期不同阶段相关的ncRNAs和mRNA。在HF的三个生长阶段，有111个长链非编码RNA（lncRNAs）、247个环状RNA（circRNAs）、97个微小RNA（miRNAs）和1168个mRNA差异表达。采用定量实时PCR验证ncRNA转录组分析结果。基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析提供了ncRNAs和mRNAs在HF周期中可能发挥的作用的信息。此外，构建lncRNA-miRNA-mRNA和circRNA-miRNA-mRNA竞争性内源RNA（ceRNA）网络以研究ncRNAs与mRNAs之间的潜在关系。发现LNC_002919和新型_circ_0026326作为ceRNAs，作为miR-320-3p的海绵参与HF周期的调控。本研究通过转录组分析全面鉴定了HF周期中的候选调控ncRNAs，突出了ncRNAs与毛发生长调控之间的可能关联。本研究为HF周期调控的系统深入研究提供了基础和新见解。
