College of Animal Science and Technology, Yangzhou University, 48 South University Ave., Yangzhou, Jiangsu 225009, PR China.
College of Animal Science and Technology, Yangzhou University, 48 South University Ave., Yangzhou, Jiangsu 225009, PR China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, PR China.
Gene. 2021 Feb 20;770:145339. doi: 10.1016/j.gene.2020.145339. Epub 2020 Dec 22.
Hair follicle (HF) development is characterized by periodic growth cycles regulated by numerous factors. We previously showed that SMAD2 might be involved in the HF growth cycle in Angora rabbits. However, its extra role in the HF growth and development remains obscure. In this study, we cloned the complete coding sequence (CDS) of the Angora rabbit SMAD2 gene. Within SMAD2 CDS, we identified the open reading frame (ORF) had a length of 1314 bp and encoding 437 amino acids. Bioinformatics analyses revealed that the SMAD2 protein is unstable and hydrophilic, and predominatelylocalizesin the cell nucleus. We identified that SMAD2 expression was elevated in the telogen phase of the during HF cycle. The knockdown and overexpression of SMAD2 could regulate HF growth and development related genes, such as WNT2, FGF2, and LEF1.Furthermore, SMAD2 may upregulate TGF-β signaling pathway-related genes, including TFDP1, E2F4, and RBL1. In conclusion, our results indicate that SMAD2 plays a vital role in HF development by regulating the TGF-β signaling pathway.
毛囊（HF）的发育特征是周期性的生长周期，由许多因素调节。我们之前曾表明，SMAD2 可能参与安哥拉兔 HF 的生长周期。然而，其在 HF 生长和发育中的额外作用尚不清楚。在这项研究中，我们克隆了安哥拉兔 SMAD2 基因的完整编码序列（CDS）。在 SMAD2 CDS 中，我们鉴定了开放阅读框（ORF）的长度为 1314bp，编码 437 个氨基酸。生物信息学分析表明，SMAD2 蛋白不稳定且亲水，主要定位于细胞核内。我们发现 SMAD2 的表达在 HF 周期的休止期升高。SMAD2 的敲低和过表达可以调节与 HF 生长和发育相关的基因，如 WNT2、FGF2 和 LEF1。此外，SMAD2 可能上调 TGF-β 信号通路相关基因，包括 TFDP1、E2F4 和 RBL1。总之，我们的结果表明，SMAD2 通过调节 TGF-β 信号通路在 HF 发育中发挥重要作用。
