Rhodes S H, Netting A G
School of Biochemistry, University of New South Wales, Kensington, Australia.
J Chromatogr. 1988 Aug 31;448(1):135-43. doi: 10.1016/s0021-9673(01)84572-6.
Triacylglycerols have been separated by normal-phase high-performance liquid chromatography (HPLC) on silica utilising a solvent system consisting of dry acetonitrile-half water saturated hexane (0.7:99.3). This solvent system is UV transparent allowing detection at 200 nm and affords a separation in which retention is primarily dependent on the number of constituent double bonds. There is also a slight separation on chainlength, the longer chainlengths being eluted first. The system is therefore complementary to currently used reversed-phase HPLC systems. Chromatograms for some polyunsaturated fats and oils are given, and the most polyunsaturated triacylglycerols from linseed oil are analysed in more detail. Data are given for the separation and quantitation of the pentafluorobenzyl esters of constituent fatty acids from these triacylglycerols by a similar normal-phase HPLC system.
三酰甘油已通过正相高效液相色谱法（HPLC）在硅胶上进行分离，使用的溶剂系统由干燥乙腈 - 半水饱和己烷（0.7:99.3）组成。该溶剂系统在紫外光下透明，允许在200nm处进行检测，并实现一种分离，其中保留主要取决于组成双键的数量。在链长方面也有轻微分离，较长链长的先被洗脱。因此，该系统与目前使用的反相HPLC系统互补。给出了一些多不饱和脂肪和油的色谱图，并对亚麻籽油中多不饱和程度最高的三酰甘油进行了更详细的分析。还给出了通过类似的正相HPLC系统对这些三酰甘油中组成脂肪酸的五氟苄基酯进行分离和定量的数据。
