Christie W W
Hannah Research Institute, U.K.
J Chromatogr. 1988 Nov 11;454:273-84. doi: 10.1016/s0021-9673(00)88620-3.
Molecular species representative of the wide range of triacylglycerols occurring in nature, ranging from relatively saturated fats such as that from sheep adipose tissue through polyunsaturated seed oils, including sunflower and linseed oils, to an oligounsaturated fish oil, have been resolved by high-performance liquid chromatography in the silver ion mode. The stationary phase consisted of an ion-exchange medium, which was a silica gel matrix with bonded sulphonic acid moieties, loaded with silver ions. The mobile phase for the more saturated fractions was a gradient of acetone into 1,2-dichloroethane-dichloromethane, then acetonitrile was introduced to elute polyunsaturated fractions. A mass detector was employed to monitor separations. Fractions were collected via a stream-splitter for identification and quantification by gas chromatography as methyl esters. Excellent resolution was obtained on the silver ion column with no contamination of fractions with silver ions, while the column was stable and retained its activity in prolonged use.
自然界中存在的种类繁多的三酰甘油的分子种类，从相对饱和的脂肪（如绵羊脂肪组织中的脂肪）到多不饱和的植物油（包括向日葵油和亚麻籽油），再到低不饱和的鱼油，已通过银离子模式的高效液相色谱法进行了分离。固定相由一种离子交换介质组成，它是一种带有键合磺酸基团的硅胶基质，并负载有银离子。对于饱和度较高的馏分，流动相是丙酮到1,2 - 二氯乙烷 - 二氯甲烷的梯度，然后引入乙腈以洗脱多不饱和馏分。使用质量检测器监测分离过程。馏分通过分流器收集，以便通过气相色谱法作为甲酯进行鉴定和定量。在银离子柱上获得了出色的分离效果，馏分没有被银离子污染，同时该柱稳定且在长期使用中保持其活性。
