Slacanin I, Marston A, Hostettmann K
Institut de Pharmacognosie et Phytochimie, Ecole de Pharmacie de l'Université de Lausanne, Switzerland.
J Chromatogr. 1988 Sep 2;448(2):265-74. doi: 10.1016/s0021-9673(01)84588-x.
A high-performance liquid chromatographic method is described for the determination of oleanane saponins in Phytolacca dodecandra (Phytolaccaceae), a plant with potent molluscicidal properties. The molluscicidal monodesmosidic saponins of the berries were determined at 254 nm as their 4-bromophenacyl derivatives, whereas the non-derivatized bidesmosidic saponins, lacking a free carboxyl group, were determined at 206 nm. A comparison of different extraction procedures showed that with cold water predominantly monodesmosidic saponins were obtained, whereas hot water gave mainly bidesmosidic (non-molluscicidal) saponins.
