Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, 50 Zhong-ling Street, Nanjing, 210014, Jiangsu, People's Republic of China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, People's Republic of China.
Arch Virol. 2020 Jun;165(6):1299-1309. doi: 10.1007/s00705-020-04567-w. Epub 2020 Apr 6.
Since late 2010, outbreaks of porcine epidemic diarrhea (PED) have been reported in the swine industry in China. A variant PEDV strain that differs from strain CV777 causes prevalent PEDV infections which commercial vaccines based on CV777 cannot provide complete protection. In this study, we designed a new vaccine based on the epidemic PEDV strain AH2012/12, adjuvanted with flagellin, a mucosal adjuvant that induces mucosal and systemic production of IgA. Three groups of pregnant sows were immunized twice, with a 14-day interval, with PEDV adjuvanted with flagellin, PEDV alone, or PBS before farrowing, and newborn piglets from each group were selected and challenged with PEDV. Immunization with this vaccine elicited high levels of IgG, IgA, and neutralizing antibodies in the serum and colostrum of sows, and newborn piglets were protected against PEDV while suckling. This study should guide the prevention and control strategies for PEDV infection, thereby reducing the losses associated with this virus.
自 2010 年末以来，中国的养猪业中报告了猪流行性腹泻（PED）的爆发。一种与 CV777 株不同的变异 PEDV 株导致了普遍的 PEDV 感染，而基于 CV777 的商业疫苗不能提供完全的保护。在本研究中，我们设计了一种基于流行的 PEDV 株 AH2012/12 的新型疫苗，并用鞭毛蛋白作为佐剂，这是一种粘膜佐剂，可诱导粘膜和全身产生 IgA。三组妊娠母猪在分娩前进行了两次免疫，间隔 14 天，分别用鞭毛蛋白佐剂的 PEDV、单独的 PEDV 或 PBS 进行免疫，然后从每组中选择新生仔猪并用 PEDV 进行攻毒。用这种疫苗免疫可在母猪的血清和初乳中诱导高水平的 IgG、IgA 和中和抗体，并且新生仔猪在哺乳期间可免受 PEDV 的侵害。本研究应指导 PEDV 感染的预防和控制策略，从而减少与该病毒相关的损失。
