Ho Thuong Thi, Trinh Vy Thai, Tran Hanh Xuan, Le Phuong Thu Thi, Nguyen Tra Thi, Hoang Hang Thu Thi, Pham Minh Dinh, Conrad Udo, Pham Ngoc Bich, Chu Ha Hoang
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Front Vet Sci. 2022 Jul 28;9:940395. doi: 10.3389/fvets.2022.940395. eCollection 2022.
Porcine epidemic diarrhea virus (PEDV) is a serious infectious causative agent in swine, especially in neonatal piglets. PEDV genotype 2 (G2) strains, particularly G2a, were the primary causes of porcine epidemic diarrhea (PED) outbreaks in Vietnam. Here, we produced a plant-based CO-26K-equivalent epitope (COE) variant from a Vietnamese highly virulent PEDV strain belonging to genotype 2a (COE/G2a) and evaluated the protective efficacy of COE/G2a-GCN4pII protein (COE/G2a-pII) in piglets against the highly virulent PEDV G2a strain following passive immunity. The 5-day-old piglets had high levels of PEDV-specific IgG antibodies, COE-IgA specific antibodies, neutralizing antibodies, and IFN-γ responses. After virulent challenge experiments, all of these piglets survived and had normal clinical symptoms, no watery diarrhea in feces, and an increase in their body weight, while all of the negative control piglets died. These results suggest that the COE/G2a-pII protein produced in plants can be developed as a promising vaccine candidate to protect piglets against PEDV G2a infection in Vietnam.
猪流行性腹泻病毒（PEDV）是猪尤其是新生仔猪的一种严重传染性病原体。PEDV基因型2（G2）毒株，特别是G2a，是越南猪流行性腹泻（PED）疫情爆发的主要原因。在此，我们从一株属于2a基因型的越南高致病性PEDV毒株中制备了一种基于植物的CO-26K等效表位（COE）变体（COE/G2a），并评估了COE/G2a-GCN4pII蛋白（COE/G2a-pII）在仔猪被动免疫后对高致病性PEDV G2a毒株的保护效果。5日龄仔猪具有高水平的PEDV特异性IgG抗体、COE-IgA特异性抗体、中和抗体和IFN-γ反应。在强毒攻击实验后，所有这些仔猪均存活，临床症状正常，粪便无水样腹泻，体重增加，而所有阴性对照仔猪均死亡。这些结果表明，植物表达的COE/G2a-pII蛋白有望开发成为一种疫苗候选物，用于保护越南仔猪免受PEDV G2a感染。
