Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269-3125, USA.
Department of Surgery, Wound Care and Hyperbaric Medicine, Hartford Health Care and the Hospital of Central Connecticut, New Britain, CT, 06050, USA.
Cell Stress Chaperones. 2020 Jul;25(4):667-677. doi: 10.1007/s12192-020-01100-5. Epub 2020 Apr 6.
We present new data on the effects of HBOT on human kidney (HK-2) cell metabolism using a SeaHorse XF Analyzer to evaluate separately the state of mitochondrial and glycolytic energy metabolism. The data are discussed in the context of the concept of cellular caloristasis networks. The information on the changes in cellular energy metabolism stimulated by HBOT presented here provides new insights into the cellular energy state and mitochondrial environment in which sHSPs function. These data will be useful in forming testable hypotheses about the functions of translocated sHSPs in human mitochondria responding to stressors.
我们使用 Seahorse XF 分析仪提供了关于高压氧治疗对人肾（HK-2）细胞代谢影响的新数据，以分别评估线粒体和糖酵解能量代谢的状态。这些数据是在细胞热稳网络概念的背景下讨论的。本文介绍的高压氧治疗刺激细胞能量代谢变化的信息提供了关于 sHSPs 发挥作用的细胞能量状态和线粒体环境的新见解。这些数据对于形成关于人类线粒体中转位 sHSPs 在应对应激源时的功能的可测试假设将是有用的。
