Marczewska Patrycja, Płonka Marlena, Rolnik Joanna, Sajewicz Mieczysław
Pesticide Quality Testing Laboratory, Institute of Plant Protection - National Research Institute, Sosnicowice, Poland.
Department of General Chemistry and Chromatography, Institute of Chemistry, University of Silesia, Katowice, Poland.
J Environ Sci Health B. 2020;55(7):599-603. doi: 10.1080/03601234.2020.1746572. Epub 2020 Apr 7.
A method was developed for the simultaneous qualitative and quantitative determination of azoxystrobin and its relevant impurity (Z)-azoxystrobin using high performance liquid chromatography with diode array detector (HPLC-DAD) in suspension concentrate (SC) pesticide formulations, with the aim of the product quality control. Method validation was realized according to SANCO/3030/99 rev. 5. The proposed method was characterized by acceptable accuracy and precision. The repeatability expressed as ratio standard deviation (%RSD) to relative standard deviation (%RSDr) was lower than 1, whereas individual recoveries were in the range of 97-103% and 90-110% for azoxystrobin and (Z)-azoxystrobin, respectively. The limit of quantification (LOQ) for the impurity ((Z)-azoxystrobin) amounted to 0.3 µg mL and was acceptable because it was lower than the maximum permitted level according to Commission Implementing Regulation (EU) No 703/2011 of 20 July 2011 for the active substance (azoxystrobin) being 25 g kg of the azoxystrobin content found. The method described in this paper is simple, precise, accurate and selective as well as represents a new and reliable way of simultaneous determination of azoxystrobin and its relevant impurity in formulated products.
为实现悬浮剂（SC）农药制剂中嘧菌酯及其相关杂质（Z）-嘧菌酯的同时定性和定量测定，建立了一种采用二极管阵列检测器的高效液相色谱法（HPLC-DAD），目的是进行产品质量控制。方法验证按照SANCO/3030/99修订版5进行。所提出的方法具有可接受的准确度和精密度。以相对标准偏差（%RSD）与相对标准偏差（%RSDr）之比表示的重复性低于1，而嘧菌酯和（Z）-嘧菌酯的单个回收率分别在97-103%和90-110%范围内。杂质（（Z）-嘧菌酯）的定量限（LOQ）为0.3μg/mL，这是可接受的，因为它低于2011年7月20日欧盟委员会实施条例（EU）No 703/2011中规定的活性物质（嘧菌酯）的最大允许水平，即嘧菌酯含量为25g/kg。本文所述方法简单、精确、准确且具有选择性，是一种同时测定制剂产品中嘧菌酯及其相关杂质的新的可靠方法。
