Wang Tengfei, Liu Jingjing, Nie Fuqiang
Division of Nanobionic Research, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou, 215123, P. R. China.
J Mater Chem B. 2018 Feb 21;6(7):1055-1065. doi: 10.1039/c7tb02631e. Epub 2018 Feb 1.
Recent advances in the field of drug screening focus on accurate, rapid and high-throughput screening methods. In our work, hydrogel inverse opal photonic crystal microspheres (HPCMs) were fabricated through a templating method and exhibited a robust and reversible response to temperature and pH. The response performance was tested under various temperature (25-55 °C) and pH (1.5-7.5) conditions and the reflective peak shifted noticeably within the visible wavelength range. Furthermore, HPCMs were used as drug delivery carriers and not only displayed high doxorubicin (DOX) drug loading but also presented thermo/pH-induced drug release properties. More importantly, these carriers were shown to be good reporters for monitoring cell viability due to their tunable colour variation. This capability was applied to H460 cell cultures with or without DOX. The structure colour of HPCMs varied in different cell culture microenvironments, and cell apoptosis was able to be distinguished. In this way, this fast, non-dyeing method for reporting cell viability in tumour cytotoxicity assays has potential in the field of drug screening and may give new insights into the use of structural colour to report results in drug screening systems.
药物筛选领域的最新进展集中在准确、快速和高通量的筛选方法上。在我们的工作中，通过模板法制备了水凝胶反蛋白石光子晶体微球（HPCMs），其对温度和pH表现出强烈且可逆的响应。在各种温度（25 - 55°C）和pH（1.5 - 7.5）条件下测试了响应性能，反射峰在可见波长范围内有明显移动。此外，HPCMs被用作药物递送载体，不仅显示出高的阿霉素（DOX）载药量，还呈现出热/ pH诱导的药物释放特性。更重要的是，由于其颜色可调节变化，这些载体被证明是监测细胞活力的良好报告物。这种能力被应用于有或没有DOX的H460细胞培养。HPCMs的结构颜色在不同的细胞培养微环境中有所变化，并且能够区分细胞凋亡。通过这种方式，这种在肿瘤细胞毒性测定中报告细胞活力的快速、非染色方法在药物筛选领域具有潜力，并且可能为在药物筛选系统中使用结构颜色报告结果提供新的见解。
