Hou Jianwen, Chen Runhai, Liu Jingchuan, Wang Haozheng, Shi Qiang, Xin Zhirong, Wong Shing-Chung, Yin Jinghua
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
J Mater Chem B. 2018 Aug 7;6(29):4792-4798. doi: 10.1039/c8tb01441h. Epub 2018 Jul 6.
We have developed a 3D smart binary polymer-brush pattern on the polymer substrate for inducing multiple cell microarrays aided by a lectin and temperature. The binary polymer-brush pattern composed of poly(N-isopropylacrylamide) (PNIPAM) and poly(d-gluconamidoethyl methacrylate) (PGAMA) brushes is fabricated by combining the photolithography technique with a surface-initiated photo-polymerization (SIPP) method. We demonstrate that well-defined binary polymer-brush patterns with high resolution are fabricated using this facile method. The patterned hierarchical PNIPAM brushes exhibit reversible switching to the adhesion of red blood cells (RBCs) induced by thermo-responsiveness. The PGAMA brush domains resist adhesion of RBCs but bind specifically with concanavalin A (Con A), forming a lectin pattern to capture RBCs in a site-specific manner. Therefore, multiple cell microarrays on a single platform are generated with the aid of Con A and temperature. This novel platform composed of a smart binary polymer-brush pattern is versatile and specific, which opens up pathways to potential applications such as microsensors, biochips and bioassays.
我们在聚合物基底上开发了一种3D智能二元聚合物刷图案，用于在凝集素和温度的辅助下诱导多细胞微阵列。由聚（N-异丙基丙烯酰胺）（PNIPAM）和聚（甲基丙烯酸d-葡糖酰胺乙酯）（PGAMA）刷组成的二元聚合物刷图案是通过将光刻技术与表面引发光聚合（SIPP）方法相结合而制备的。我们证明，使用这种简便方法可以制备出具有高分辨率的明确二元聚合物刷图案。图案化的分级PNIPAM刷表现出由热响应诱导的对红细胞（RBC）粘附的可逆切换。PGAMA刷域可抵抗RBC的粘附，但与伴刀豆球蛋白A（Con A）特异性结合，形成凝集素图案以位点特异性方式捕获RBC。因此，借助Con A和温度在单个平台上生成了多细胞微阵列。这种由智能二元聚合物刷图案组成的新型平台具有通用性和特异性，为微传感器、生物芯片和生物测定等潜在应用开辟了道路。
