State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
Polymer Materials Research Center, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin, 150001, P. R. China.
Macromol Rapid Commun. 2018 Mar;39(6):e1700572. doi: 10.1002/marc.201700572. Epub 2018 Jan 4.
A versatile platform allowing capture and detection of normal and dysfunctional cells on the same patterned surface is important for accessing the cellular mechanism, developing diagnostic assays, and implementing therapy. Here, an original and effective method for fabricating binary polymer brushes pattern is developed for controlled cell adhesion. The binary polymer brushes pattern, composed of poly(N-isopropylacrylamide) (PNIPAAm) and poly[poly(ethylene glycol) methyl ether methacrylate] (POEGMA) chains, is simply obtained via a combination of surface-initiated photopolymerization and surface-activated free radical polymerization. This method is unique in that it does not utilize any protecting groups or procedures of backfilling with immobilized initiator. It is demonstrated that the precise and well-defined binary polymer patterns with high resolution are fabricated using this facile method. PNIPAAm chains capture and release cells by thermoresponsiveness, while POEGMA chains possess high capability to capture dysfunctional cells specifically, inducing a switch of normal red blood cells (RBCs) arrays to hemolytic RBCs arrays on the pattern with temperature. This novel platform composed of binary polymer brush pattern is smart and versatile, which opens up pathways to potential applications as microsensors, biochips, and bioassays.
一个通用的平台，能够在同一图案化表面上捕获和检测正常和功能失调的细胞，对于研究细胞机制、开发诊断检测方法和实施治疗都很重要。在这里，我们开发了一种原始而有效的方法，用于制造用于控制细胞黏附的二元聚合物刷图案。该二元聚合物刷图案由聚（N-异丙基丙烯酰胺）（PNIPAAm）和聚[聚（乙二醇）甲基醚甲基丙烯酸酯]（POEGMA）链组成，通过表面引发光聚合和表面激活自由基聚合的组合简单地获得。这种方法的独特之处在于它不使用任何保护基团或固定化引发剂的回填程序。结果表明，使用这种简单的方法可以制造出具有高分辨率的精确和定义良好的二元聚合物图案。PNIPAAm 链通过温度响应性捕获和释放细胞，而 POEGMA 链具有特异性捕获功能失调细胞的高能力，导致图案上的正常红细胞（RBC）阵列向溶血 RBC 阵列的转变。这种由二元聚合物刷图案组成的新型平台既智能又多功能，为微传感器、生物芯片和生物测定等潜在应用开辟了道路。
