Qiu Jiajun, Liu Lu, Chen Baohui, Qiao Yuqin, Cao Huiliang, Zhu Hongqin, Liu Xuanyong
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
J Mater Chem B. 2018 Apr 7;6(13):2004-2012. doi: 10.1039/c8tb00162f. Epub 2018 Mar 21.
Implant failure of titanium and its alloys still occurs due to insufficient osseointegration and implant associated infections. Therefore, titanium implants with enhanced osseointegration and antibacterial activities are desirable. To achieve this goal, graphene oxide (GO) as a dual zinc/magnesium (Zn/Mg) ion carrier and release platform was constructed on the titanium surface by cathode electrophoresis deposition. Rat bone mesenchymal stem cells were used to investigate the osteogenic activity. Gram-negative E. coli was utilized to assess the antibacterial activities. Results show that GO co-doped with Mg and Zn ions with a sustained and slow release exhibited the best osteogenic activity among all the samples by stimulating the expressions of osteogenesis related genes containing alkaline phosphatase (ALP), type I collagen (Col-I), osteocalcin (OCN), and osteopontin (OPN), and by promoting the alkaline phosphatase activity and extracellular matrix mineralization. The existence of GO endowed titanium with superior antibacterial activities against Gram-negative E. coli. Due to the synergistic effects of GO, Mg and Zn ions, a titanium surface with enhanced osseointegration and antibacterial activities was achieved.
由于骨整合不足和植入物相关感染，钛及其合金的植入失败仍然会发生。因此，需要具有增强骨整合和抗菌活性的钛植入物。为了实现这一目标，通过阴极电泳沉积在钛表面构建了作为双锌/镁（Zn/Mg）离子载体和释放平台的氧化石墨烯（GO）。使用大鼠骨髓间充质干细胞来研究成骨活性。利用革兰氏阴性大肠杆菌来评估抗菌活性。结果表明，共掺杂Mg和Zn离子并具有持续缓慢释放特性的GO通过刺激包含碱性磷酸酶（ALP）、I型胶原（Col-I）、骨钙素（OCN）和骨桥蛋白（OPN）的成骨相关基因的表达，并通过促进碱性磷酸酶活性和细胞外基质矿化，在所有样品中表现出最佳的成骨活性。GO的存在赋予钛对革兰氏阴性大肠杆菌卓越的抗菌活性。由于GO、Mg和Zn离子的协同作用，实现了具有增强骨整合和抗菌活性的钛表面。
