Department of Orthopedics, The First Affiliated Hospital of Air Force Military Medical University, Xi'an, People's Republic of China.
Department of Joint Surgery, Hong-Hui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, People's Republic of China.
Int J Nanomedicine. 2021 Oct 27;16:7249-7268. doi: 10.2147/IJN.S321800. eCollection 2021.
With increases in implant infections, the search for antibacterial and biofilm coatings has become a new interest for orthopaedists and dentists. In recent years, graphene oxide (GO) has been extensively studied for its superior antibacterial properties. However, most of these studies have focused on solutions and there are few antibacterial studies on metal surfaces, especially the surfaces of cobalt-chromium-molybdenum (CoCrMo) alloys. ε-Poly-L-lysine (ε-PLL), as a novel food preservative, has a spectrum of antimicrobial activity; however, its antimicrobial activity after coating an implant surface is not clear.
In this study, for the first time, a two-step electrodeposition method was used to coat GO and ε-PLL on the surface of a CoCrMo alloy. Its antibacterial and antibiofilm properties against and were then studied.
The results show that the formation of bacteria and biofilms on the coating surface was significantly inhibited, GO and ε-PLL composite coatings had the best antibacterial and antibiofilm effects, followed by ε-PLL and GO coatings. In terms of classification, the coatings are anti-adhesive and contact-killing/inhibitory surfaces. In addition to oxidative stress, physical damage to GO and electrostatic osmosis of ε-PLL are the main antibacterial and antibiofilm mechanisms.
This is the first study that GO and ε-PLL coatings were successfully prepared on the surface of CoCrMo alloy by electrodeposition. It provides a promising new approach to the problem of implant infection in orthopedics and stomatology.
随着植入物感染的增加，寻找具有抗菌和抗生物膜性能的涂层已成为骨科医生和牙医的新关注点。近年来，氧化石墨烯（GO）因其卓越的抗菌性能而受到广泛研究。然而，这些研究大多集中在溶液上，而关于金属表面（尤其是钴铬钼（CoCrMo）合金表面）的抗菌研究较少。ε-聚赖氨酸（ε-PLL）作为一种新型食品防腐剂，具有广谱抗菌活性；然而，其在涂覆植入物表面后的抗菌活性尚不清楚。
本研究首次采用两步电沉积法在 CoCrMo 合金表面涂覆 GO 和 ε-PLL。然后研究了其对 和 的抗菌和抗生物膜性能。
结果表明，涂层表面的细菌和生物膜形成明显受到抑制，GO 和 ε-PLL 复合涂层具有最佳的抗菌和抗生物膜效果，其次是 ε-PLL 和 GO 涂层。就分类而言，这些涂层具有抗黏附性和接触杀伤/抑制性表面。除氧化应激外，GO 的物理损伤和 ε-PLL 的静电渗透是其主要的抗菌和抗生物膜机制。
这是首次通过电沉积成功在 CoCrMo 合金表面制备 GO 和 ε-PLL 涂层的研究。它为解决骨科和口腔医学领域的植入物感染问题提供了一种很有前途的新方法。
