Zhang Lin, Yang Xiao-Quan, An Jie, Zhao Sun-Duo, Zhao Tian-Yu, Tan Fang, Cao Yuan-Cheng, Zhao Yuan-Di
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Key Laboratory of Biomedical Photonics (HUST, Ministry of Education), Collaborative Innovation Center for Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China.
J Mater Chem B. 2018 May 7;6(17):2574-2583. doi: 10.1039/c7tb02643a. Epub 2018 Jan 4.
In this paper, gold nanorods and InP/ZnS quantum dots were encapsulated together in a silica medium, and the targeting molecular peptide c(RGDfC) was further connected after surface modification with PEG and PEG derivatives to prepare a multifunctional Au@QD@SiO/PEG-c(RGDfC) probe. Dynamic Light Scattering showed that the probe size was about 215.01 ± 2.72 nm, and its dispersibility was good. In in vitro experiments when the concentration was as high as 200 μg mL, the activity of the cells was still 85% due to low toxicity. In vivo experiments showed that the probe had excellent tumor targeting, X-ray computed tomography (CT) imaging and fluorescence imaging capabilities. The experiments revealed that the probe had a long blood circulation time (T = 7.78 h) in mice. Biochemical analysis, liver enzyme analysis and histomorphological analysis after probe injection showed that the probe had no obvious side effects on the normal functions of the main organs, indicating good biosafety. In vivo imaging experiments showed that 6 d after intravenous injection, the tumor sites of a HeLa tumor-bearing nude mice positive group presented obvious fluorescence and CT signals, indicating that the prepared nanoprobe had good tumor targeting dual-mode imaging capabilities and therefore showed great potential in biomedical imaging applications, especially the diagnosis of cancer.
在本文中，金纳米棒和InP/ZnS量子点被共同包裹在二氧化硅介质中，并且在经过聚乙二醇（PEG）及其衍生物的表面修饰后，进一步连接靶向分子肽c(RGDfC)，以制备多功能Au@QD@SiO/PEG-c(RGDfC)探针。动态光散射表明，该探针尺寸约为215.01±2.72 nm，且分散性良好。在体外实验中，当浓度高达200 μg/mL时，由于毒性较低，细胞活性仍为85%。体内实验表明，该探针具有优异的肿瘤靶向性、X射线计算机断层扫描（CT）成像和荧光成像能力。实验显示，该探针在小鼠体内具有较长的血液循环时间（T = 7.78 h）。注射探针后的生化分析、肝酶分析和组织形态学分析表明，该探针对主要器官的正常功能无明显副作用，表明其具有良好的生物安全性。体内成像实验表明，静脉注射6 d后，荷HeLa瘤裸鼠阳性组的肿瘤部位呈现明显的荧光和CT信号，表明所制备的纳米探针具有良好的肿瘤靶向双模成像能力，因此在生物医学成像应用，尤其是癌症诊断方面显示出巨大潜力。
