Huang Lin, Liu Xin-Hua, Zhang Xiao-Hong, Tan Lei, Liu Chuan-Jun
Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Science, Wuhan University, Wuhan, Hubei 430072, P. R. China.
J Mater Chem B. 2018 Nov 14;6(42):6831-6841. doi: 10.1039/c8tb02010h. Epub 2018 Oct 11.
Bacterial infection is posing a great threat to human life, and constructing a platform to capture or kill the bacteria attached on a material surface is of particular significance. Herein, a nano-topographic material surface (SiNW-p-ppix@CDm) has been successfully synthesized based on silicon nanowire (SiNW) arrays modified with a random copolymer, which was decorated with photosensitive protoporphyrin IX (ppix) and β-CD-mannose (CDm). The as-prepared surface exhibits a highly efficient bacterial capture, which is based on the co-capture function between the SiNW topographic surface and the bacterial attachment molecule CDm, and sterilization of ppix under irradiation by 630 nm light. The Gram-negative bacterium Escherichia coli (E. coli) was adopted to evaluate the surface bactericidal efficiency. Finally, the killing efficiency can be proved to be 96.7% from fluorescence microscopy after staining with the live/dead bacterial viability kit. The reason for sterilization is that the bacterial cell wall had been split by the reactive oxygen species (ROS), which could be demonstrated via scanning electronic microscopy (SEM). This functional substrate could be used for bacterial enrichment apparatus such as used in water quality monitoring, and even in constructing clinical antibacterial materials.
细菌感染对人类生命构成了巨大威胁，构建一个能够捕获或杀死附着在材料表面细菌的平台具有特别重要的意义。在此，基于用随机共聚物修饰的硅纳米线（SiNW）阵列成功合成了一种纳米拓扑材料表面（SiNW-p-ppix@CDm），该阵列装饰有光敏原卟啉IX（ppix）和β-CD-甘露糖（CDm）。所制备的表面表现出高效的细菌捕获能力，这基于SiNW拓扑表面与细菌附着分子CDm之间的共捕获功能，以及在630 nm光照射下ppix的杀菌作用。采用革兰氏阴性菌大肠杆菌（E. coli）来评估表面杀菌效率。最后，在用活/死细菌活力试剂盒染色后，通过荧光显微镜可证明杀灭效率为96.7%。杀菌的原因是细菌细胞壁已被活性氧（ROS）裂解，这可通过扫描电子显微镜（SEM）证实。这种功能性底物可用于水质监测等细菌富集装置，甚至可用于构建临床抗菌材料。
