Wang Jie, Chen Dong, Yang Kaitong, Song Chenglong, Chen Wenzhao, Chang Peng, Shen Danfeng, Zhu Zhiqiang
Institute for Advanced Materials, School of Material Science and Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
School of Biomedical Sciences, Suzhou Chien-shiung Institute of Technology, Suzhou, 215411, People's Republic of China.
Sci Rep. 2024 Dec 28;14(1):31273. doi: 10.1038/s41598-024-82738-4.
Over the past decades, bacterial infections resulting from the misuse of antibiotics have garnered significant attention. Among the alternative antibacterial strategies, photodynamic therapy (PDT) has emerged as a promising non-antibiotic approach. However, persistent bacterial biofilms, particularly those composed of gram-negative bacteria with their protective outer membranes, have exhibited remarkable resilience to PDT. To address this challenge, we have developed an antibacterial composite material, silver nanoparticles coated-copper cysteamine (Cu-Cy-PEG@AgNPs), which demonstrates remarkable antibacterial activity against both gram-positive and gram-negative bacteria. Specifically, under UV irradiation, Cu-Cy-PEG@AgNPs achieves a sterilization efficiency of approximately 100% at a low concentration of 25 µg/mL. The incorporation of silver nanoparticles significantly enhances the antibacterial performance of Cu-Cy, effectively eradicating persistent bacterial biofilm infections. Moreover, Cu-Cy-PEG@AgNPs exhibits excellent biocompatibility with L929 cells, indicating its potential for use in relevant applications to combat bacterial infections.
在过去几十年中，抗生素滥用导致的细菌感染已引起广泛关注。在替代抗菌策略中，光动力疗法（PDT）已成为一种有前景的非抗生素方法。然而，持久性细菌生物膜，尤其是那些由具有保护性外膜的革兰氏阴性菌组成的生物膜，对PDT表现出显著的抗性。为应对这一挑战，我们开发了一种抗菌复合材料，即铜半胱胺包覆银纳米颗粒（Cu-Cy-PEG@AgNPs），它对革兰氏阳性菌和革兰氏阴性菌均表现出显著的抗菌活性。具体而言，在紫外线照射下，Cu-Cy-PEG@AgNPs在25μg/mL的低浓度下实现了约100%的杀菌效率。银纳米颗粒的加入显著增强了Cu-Cy的抗菌性能，有效根除了持久性细菌生物膜感染。此外，Cu-Cy-PEG@AgNPs与L929细胞表现出优异的生物相容性，表明其在对抗细菌感染的相关应用中的潜力。
