Gai Panpan, Zhang Shuxia, Yu Wen, Li Haiyin, Li Feng
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, P. R. China.
J Mater Chem B. 2018 Nov 14;6(42):6842-6847. doi: 10.1039/c8tb02286k. Epub 2018 Oct 12.
Herein, a light-driven self-powered biosensor based on a photoelectrochemical enzymatic fuel cell (PEFC) was proposed for ultrasensitive organophosphate pesticide (OP) detection. To construct this fuel cell, poly(3,4-ethylenedioxythiophene)-sensitized CdS quantum dot (PEDOT/CdS) photoanode and multiwalled carbon nanotubes/gold nanoparticals/bilirubin oxidase (CNTs/AuNPs/BOD) biocathode were elaborately designed. Initially, the enzyme acetylcholinesterase (AChE) immobilized on the CdS/PEDOT photoanode could hydrolyze acetylthiocholine iodide (ATCh) into thiocholine as the electron donor to enhance the charge separation efficiency; thus, PEFC produced relatively high open circuit voltage (E) upon illumination. OPs could inhibit the AChE enzymatic catalytic activity on hydrolysis, and in this case, a smaller E obtained. Thus, based on the constructed PEFC, self-powered biosensing of OPs was realized, and the detection limit was as low as 0.012 ng mL (S/N = 3), which was comparable or superior to those of the reported methods. In this study, we not only constructed a facile, ultrasensitive and low-cost sensing platform for OP detection, but also provided an ingenious idea for designing light-driven self-powered biosensing based on PEFC.
在此，提出了一种基于光电化学酶燃料电池（PEFC）的光驱动自供电生物传感器，用于超灵敏检测有机磷农药（OP）。为构建该燃料电池，精心设计了聚（3,4-乙撑二氧噻吩）敏化的硫化镉量子点（PEDOT/CdS）光阳极和多壁碳纳米管/金纳米颗粒/胆红素氧化酶（CNTs/AuNPs/BOD）生物阴极。最初，固定在CdS/PEDOT光阳极上的乙酰胆碱酯酶（AChE）可将碘化硫代乙酰胆碱（ATCh）水解为硫代胆碱作为电子供体，以提高电荷分离效率；因此，PEFC在光照下产生相对较高的开路电压（E）。OPs可抑制AChE对水解的酶催化活性，此时获得的E较小。因此，基于构建的PEFC实现了OPs的自供电生物传感，检测限低至0.012 ng/mL（S/N = 3），与已报道的方法相当或更优。在本研究中，我们不仅构建了一个用于OP检测的简便、超灵敏且低成本的传感平台，还为基于PEFC设计光驱动自供电生物传感提供了一个巧妙的思路。
