Kong Ting Ting, Zhao Zheng, Li Ying, Wu Fei, Jin Tuo, Tang Ben Zhong
Center for BioDelivery Sciences, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
J Mater Chem B. 2018 Oct 7;6(37):5986-5991. doi: 10.1039/c8tb01390j. Epub 2018 Sep 7.
A new class of biosensor molecules evoking fluorescent emission by rotation-restricted binding with bacteria was examined for its applicability in detecting live bacteria instantly. The fluorogens possessed multiple tetraphenylethene (TPE)-cored boronic acids to oligomerize through complexation with cis-diols on bacterial surfaces, resulting in aggregation-induced emission (AIE). The fluorogen having two boronic acid units discriminated between live and dead bacteria by showing AIE activity only with the latter. Live bacteria were instantly detected by consequent treatment with reagents of three and four di-boronates (which showed AIE activity with both live and dead bacteria). This phenomenon may lead to a practical method for live bacteria detection.
研究了一类新型生物传感器分子，该分子通过与细菌的旋转受限结合引发荧光发射，以考察其在即时检测活细菌方面的适用性。荧光团含有多个以四苯乙烯（TPE）为核心的硼酸，通过与细菌表面的顺式二醇络合而寡聚，从而产生聚集诱导发光（AIE）。具有两个硼酸单元的荧光团仅对死细菌表现出AIE活性，从而区分活细菌和死细菌。通过随后用三硼酸酯和四硼酸酯试剂处理（这两种试剂对活细菌和死细菌均表现出AIE活性），可即时检测活细菌。这一现象可能会带来一种实用的活细菌检测方法。
