将金纳米粒子与聚合酶链反应相结合，构建用于检测与健康相关的 DNA 和蛋白质的比色传感平台。
The Integration of Gold Nanoparticles with Polymerase Chain Reaction for Constructing Colorimetric Sensing Platforms for Detection of Health-Related DNA and Proteins.
机构信息
Institute of Medical Research, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an 710072, China.
Research & Development Institute of Northwestern Polytechnical University in Shenzhen, 45 South Gaoxin Road, Shenzhen 518057, China.
出版信息
Biosensors (Basel). 2022 Jun 16;12(6):421. doi: 10.3390/bios12060421.
Polymerase chain reaction (PCR) is the standard tool in genetic information analysis, and the desirable detection merits of PCR have been extended to disease-related protein analysis. Recently, the combination of PCR and gold nanoparticles (AuNPs) to construct colorimetric sensing platforms has received considerable attention due to its high sensitivity, visual detection, capability for on-site detection, and low cost. However, it lacks a related review to summarize and discuss the advances in this area. This perspective gives an overview of established methods based on the combination of PCR and AuNPs for the visual detection of health-related DNA and proteins. Moreover, this work also addresses the future trends and perspectives for PCR-AuNP hybrid biosensors.
聚合酶链式反应（PCR）是遗传信息分析的标准工具，PCR 的理想检测优点已扩展到与疾病相关的蛋白质分析。最近，由于其高灵敏度、可视化检测、现场检测能力和低成本，PCR 与金纳米粒子（AuNPs）结合构建比色传感平台受到了相当大的关注。然而，目前缺乏相关的综述来总结和讨论该领域的进展。本文综述了基于 PCR 和 AuNPs 结合的用于检测与健康相关的 DNA 和蛋白质的可视化方法。此外，本工作还探讨了 PCR-AuNP 杂化生物传感器的未来趋势和展望。
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