Trachsel Lucca, Broguiere Nicolas, Rosenboom Jan-Georg, Zenobi-Wong Marcy, Benetti Edmondo M
Tissue Engineering + Biofabrication, Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland.
J Mater Chem B. 2018 Dec 14;6(46):7568-7572. doi: 10.1039/c8tb02382d. Epub 2018 Oct 31.
Molecularly designed, random copolymer-bioconjugates based on poly(2-methyl-2-oxazoline) (PMOXA) and poly(2-ethyl-2-oxazoline) (PEOXA) are crosslinked in the presence of sortase A (SA) and human articular chondrocytes (hACs), to yield cellularized polymer networks. Their gelation kinetics and mechanical properties are finely tuned by varying the concentration of SA, while a cell viability >90% is achieved after several weeks of culture, even in the absence of any cell-adhesive cue.
基于聚（2-甲基-2-恶唑啉）（PMOXA）和聚（2-乙基-2-恶唑啉）（PEOXA）的分子设计随机共聚物生物共轭物在分选酶A（SA）和人关节软骨细胞（hACs）存在下交联，以产生细胞化聚合物网络。通过改变SA的浓度可精细调节其凝胶化动力学和机械性能，即使在没有任何细胞粘附线索的情况下，培养数周后细胞活力仍可达到>90%。
