Qiao Min, Liu Xun, Song Jin-Wei, Yang Ting, Chen Ming-Li, Wang Jian-Hua
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
J Mater Chem B. 2018 Dec 14;6(46):7703-7709. doi: 10.1039/c8tb02221f. Epub 2018 Nov 6.
Tryptophan (Trp) modified aminated mesoporous silica nanoparticles (AMSNs), shortened to Trp-AMSNs, are prepared via covalent binding. The obtained Trp-AMSNs exhibit a uniform size of ca. 83 nm, a mesopore diameter of ca. 2.6 nm, along with a pore volume of 0.439 cm g. It is demonstrated that Trp-AMSNs selectively adsorb ovalbumin (Ova) from complex biological matrices. At pH 5.0, 1.0 mg of Trp-AMSNs produces an adsorption efficiency of 96% for 100 mg L Ova in 1.0 mL of solution. An adsorption capacity of 1240.3 mg g is derived for Ova, which is much improved with respect to that of the native AMSNs. The retained Ova could be readily recovered by a sodium dodecyl sulfate (SDS) solution (0.5%, m/v), providing a recovery of 71.2%. Trp-AMSNs are further applied for the isolation of Ova from a protein mixture (with a molar ratio of ovalbumin/lysozyme of 1 : 10) and an egg-white sample. High-purity Ova is obtained, as demonstrated by SDS-PAGE assay results.
色氨酸（Trp）修饰的胺化介孔二氧化硅纳米颗粒（AMSNs），简称为Trp-AMSNs，通过共价结合制备。所获得的Trp-AMSNs呈现出约83 nm的均匀尺寸、约2.6 nm的中孔直径以及0.439 cm³ g的孔体积。结果表明，Trp-AMSNs能从复杂的生物基质中选择性吸附卵清蛋白（Ova）。在pH 5.0时，1.0 mg的Trp-AMSNs对1.0 mL溶液中100 mg/L的Ova产生96%的吸附效率。得出Ova的吸附容量为1240.3 mg/g，相对于天然AMSNs有很大提高。保留的Ova可以很容易地用十二烷基硫酸钠（SDS）溶液（0.5%，m/v）回收，回收率为71.2%。Trp-AMSNs进一步应用于从蛋白质混合物（卵清蛋白/溶菌酶的摩尔比为1∶10）和蛋清样品中分离Ova。如SDS-PAGE分析结果所示，获得了高纯度的Ova。
