Qiao Min, Wang Meng-Meng, Chen Ming-Li, Wang Jian-Hua
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Anal Chim Acta. 2021 Mar 1;1148:238176. doi: 10.1016/j.aca.2020.12.051. Epub 2021 Jan 5.
Porous polymeric microspheres, poly(styrene-divinyl benzene, PSDVB)-poly(ethylene glycol monoallyl ether, PEGMAE), termed as PSDVB-PEGMAE, are prepared via double emulsion interfacial polymerization strategy. PSDVB-PEGMAE microspheres exhibit a mean diameter of 2.98 μm, and possess heterogeneous porous structure with a pore volume of 0.354 cm g and a pore size of 34.3 nm. PEGMAE moiety is identified on the external surface of the microspheres, while both PSDVB and PEGMAE moieties are found in the interior pores. The PSDVB-PEGMAE microspheres possess favorable selectivity towards the adsorption of conalbumin (ConA) through hydrogen-bonding and hydrophobic interactions, via surface and inter-pore adsorption. At pH 6, an adsorption capacity of 171.9 mg g is achieved for ConA. The captured ConA may be readily recovered by stripping with a cetane trimethyl ammonium bromide (CTAB) solution (0.1%, m/v). The microspheres are further used for the isolation of ConA from egg white, deriving high purity ConA as demonstrated by SDS-PAGE assay.
通过双乳液界面聚合策略制备了多孔聚合物微球，即聚（苯乙烯 - 二乙烯基苯，PSDVB）-聚（乙二醇单烯丙基醚，PEGMAE），简称为PSDVB - PEGMAE。PSDVB - PEGMAE微球的平均直径为2.98μm，具有非均相多孔结构，孔体积为0.354 cm³/g，孔径为34.3 nm。在微球的外表面鉴定出PEGMAE部分，而在内部孔隙中同时发现了PSDVB和PEGMAE部分。PSDVB - PEGMAE微球通过表面和孔间吸附，通过氢键和疏水相互作用对伴刀豆球蛋白（ConA）的吸附具有良好的选择性。在pH 6时，对ConA的吸附容量达到171.9 mg/g。捕获的ConA可以通过用十六烷基三甲基溴化铵（CTAB）溶液（0.1%，m/v）洗脱而容易地回收。这些微球进一步用于从蛋清中分离ConA，如SDS - PAGE分析所示，可获得高纯度的ConA。
