miR-34a 可能作为创伤性脊髓损伤后缓解的潜在生物标志物。
MiR-34a may Function as Potential Biomarker for Remission after Traumatic Spinal Cord Injury.
出版信息
Clin Lab. 2020 Apr 1;66(4). doi: 10.7754/Clin.Lab.2019.190916.
BACKGROUND
Emerging evidence has manifested that many microRNAs (miRNAs) exert crucial roles in the responses and remission process of traumatic spinal cord injury (TSCI). The present study aimed to investigate clinical significance of miR-34a concerning the assessment of neurological remission and prognosis after TSCI.
METHODS
We examined the serum levels of miR-34a in patients with TSCI and healthy controls through real-time polymerase chain reaction (RT-qPCR) assay. Then, receiver operating characteristic (ROC) curve was used to determine the diagnostic value of miR-34a in TSCI. Finally, we detected the expression levels of miR-34a from serum samples over a 12-week period.
RESULTS
The results of our study demonstrated that miR-34a was significantly down-regulated in TSCI patients compared with healthy controls. miR-34a may function a potential biomarker for TSCI diagnosis with an area under curve (AUC) of 0.8020. The expression of miR-34a was increased in the remission group compared to the non-remission group after 12 weeks post-injury. The expression of miR-34a was negatively related to TNF-alpha, IL-6, and IL-8.
CONCLUSIONS
Measuring serum expression level of miR-34a over time may be used in tracking the process and neurological remission of TSCI.
背景
越来越多的证据表明，许多 microRNAs（miRNAs）在创伤性脊髓损伤（TSCI）的反应和缓解过程中发挥着关键作用。本研究旨在探讨 miR-34a 在评估 TSCI 后神经恢复和预后方面的临床意义。
方法
我们通过实时聚合酶链反应（RT-qPCR）检测了 TSCI 患者和健康对照者血清中的 miR-34a 水平。然后，使用接收者操作特征（ROC）曲线确定 miR-34a 在 TSCI 中的诊断价值。最后，我们检测了血清样本在 12 周内 miR-34a 的表达水平。
结果
研究结果表明，与健康对照组相比，TSCI 患者的 miR-34a 水平显著下调。miR-34a 可能是 TSCI 诊断的潜在生物标志物，曲线下面积（AUC）为 0.8020。与非缓解组相比，在损伤后 12 周时，缓解组的 miR-34a 表达增加。miR-34a 的表达与 TNF-α、IL-6 和 IL-8 呈负相关。
结论
随着时间的推移测量血清中 miR-34a 的表达水平，可能用于跟踪 TSCI 的过程和神经恢复。
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