Corral Jordi, Pérez-Varela María, Barbé Jordi, Aranda Jesús
Departament de Genètica i Microbiologia, Facultat de Biociènces, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Virulence. 2020 Dec;11(1):315-326. doi: 10.1080/21505594.2020.1748923.
is a nosocomial pathogen that causes multi-drug resistant infections mainly in immunocompromised patients. Although this gram-negative species lacks flagella, it is able to move over wet surfaces through a not well characterized type of movement known as surface-associated motility. In this study we demonstrate through the inactivation of the gene (coding a CheW-like protein) and (coding a DNA damage repair and recombination protein) that both genes are involved in the surface-associated motility and chemotaxis of ATCC 17978 strain. In addition, we also point out that the lack of either RecA or CheW-like proteins reduces its virulence in the and the animal models. Furthermore, we show through co-immunoprecipitation assays that the CheW-like protein and RecA interact and that this interaction is abolished by the introduction of the mutation S97A in one of the domains of CheW-like protein that is structurally conserved in and necessary for the RecA-CheW interaction in this bacterial species. Finally, we show that the replacement of the wild-type CheW-like protein by that presenting the S97A mutation impairs surface-associated motility, chemotaxis and virulence of strain ATCC 17978.
是一种医院病原体，主要在免疫功能低下的患者中引起多重耐药感染。尽管这种革兰氏阴性菌缺乏鞭毛，但它能够通过一种特征不明的运动方式，即表面相关运动，在潮湿表面上移动。在本研究中，我们通过使基因（编码一种类CheW蛋白）和（编码一种DNA损伤修复和重组蛋白）失活，证明这两个基因都参与了ATCC 17978菌株的表面相关运动和趋化性。此外，我们还指出，缺乏RecA或类CheW蛋白会降低其在和动物模型中的毒力。此外，我们通过免疫共沉淀试验表明，类CheW蛋白和RecA相互作用，并且通过在类CheW蛋白的一个结构域中引入S97A突变，这种相互作用被消除，该结构域在中是保守的，并且对于该细菌物种中RecA-CheW相互作用是必需的。最后，我们表明，用具有S97A突变的蛋白替代野生型类CheW蛋白会损害ATCC 17978菌株的表面相关运动、趋化性和毒力。
