Department of Dermatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Department of Dermatology, Kawasaki Medical School, Okayama, Japan.
J Med Virol. 2020 Dec;92(12):3689-3696. doi: 10.1002/jmv.25811. Epub 2020 Apr 25.
Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut-off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell-free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell-free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.
EB 病毒（EBV）载量在移植后淋巴组织增生性疾病和慢性活动性 EBV 感染中增加。在本报告中，我们分析了患有水疱样疹（HV）和/或蚊虫叮咬过敏（HMB）患者外周血单个核细胞（PBMC）和血浆中的 EBV DNA 载量，以了解 EBV DNA 载量的临床意义。所有 30 例患者的 PBMC 均超过截止值显示出高 DNA 载量。在 16 个血浆样本中，从患有噬血细胞性淋巴组织细胞增生症（HLH）的患者获得的两个样本中，DNA 载量极高。血浆中无细胞游离 DNA 的量与血清乳酸脱氢酶水平相关，与血小板计数呈负相关。这些结果表明，PBMC 中的 EBV DNA 载量可作为 HV 和 HMB 的诊断指标之一，而血浆中 EBV 游离 DNA 的显著升高可能与 HLH 中观察到的细胞溶解有关。
