Queensland University of Technology, School of Biomedical Sciences, Brisbane, Australia.
Queensland University of Technology, Institute of Health and Biomedical Innovation, Brisbane, Australia.
Am J Physiol Endocrinol Metab. 2020 Jun 1;318(6):E1022-E1037. doi: 10.1152/ajpendo.00433.2019. Epub 2020 Apr 7.
Proteomics offers the opportunity to identify and quantify many proteins and to explore how they correlate and interact with each other in biological networks. This study aimed to characterize changes in the muscle proteome during the destruction, repair, and early-remodeling phases after impact trauma in male Wistar rats. Muscle tissue was collected from uninjured control rats and rats that were euthanized between 6 h and 14 days after impact injury. Muscle tissue was analyzed using unbiased, data-independent acquisition LC-MS/MS. We identified 770 reviewed proteins in the muscle tissue, 296 of which were differentially abundant between the control and injury groups ( ≤ 0.05). Around 50 proteins showed large differences (≥10-fold) or a distinct pattern of abundance after injury. These included proteins that have not been identified previously in injured muscle, such as ferritin light chain 1, fibrinogen γ-chain, fibrinogen β-chain, osteolectin, murinoglobulin-1, T-kininogen 2, calcium-regulated heat-stable protein 1, macrophage-capping protein, retinoid-inducible serine carboxypeptidase, ADP-ribosylation factor 4, Thy-1 membrane glycoprotein, and ADP-ribosylation factor-like protein 1. Some proteins increased between 6 h and 14 days, whereas other proteins increased in a more delayed pattern at 7 days after injury. Bioinformatic analysis revealed that various biological processes, including regulation of blood coagulation, fibrinolysis, regulation of wound healing, tissue regeneration, acute inflammatory response, and negative regulation of the immune effector process, were enriched in injured muscle tissue. This study advances the understanding of early muscle healing after muscle injury and lays a foundation for future mechanistic studies on interventions to treat muscle injury.
蛋白质组学提供了鉴定和定量许多蛋白质的机会，并探索了它们在生物网络中如何相互关联和相互作用。本研究旨在描述雄性 Wistar 大鼠冲击伤后破坏、修复和早期重塑阶段肌肉蛋白质组的变化。收集未受伤对照大鼠和冲击伤后 6 小时至 14 天处死大鼠的肌肉组织。使用无偏、数据独立获取的 LC-MS/MS 分析肌肉组织。我们在肌肉组织中鉴定了 770 个经审查的蛋白质，其中 296 个在对照组和损伤组之间丰度差异显著（≤0.05）。约 50 种蛋白质在损伤后表现出较大差异（≥10 倍）或丰度模式明显改变。其中包括以前在损伤肌肉中未鉴定到的蛋白质，如铁蛋白轻链 1、纤维蛋白原γ链、纤维蛋白原β链、骨粘连蛋白、鼠球蛋白-1、T-激肽原 2、钙调节热稳定蛋白 1、巨噬细胞封顶蛋白、视黄醇诱导丝氨酸羧肽酶、ADP-核糖基化因子 4、Thy-1 膜糖蛋白和 ADP-核糖基化因子样蛋白 1。一些蛋白质在 6 小时至 14 天之间增加，而其他蛋白质在损伤后 7 天以更延迟的模式增加。生物信息学分析显示，各种生物学过程，包括血液凝固、纤维蛋白溶解、伤口愈合、组织再生、急性炎症反应和免疫效应物过程的负调控，在损伤肌肉组织中富集。本研究加深了对肌肉损伤后早期肌肉愈合的理解，为未来治疗肌肉损伤的干预措施的机制研究奠定了基础。
