Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Canada.
Viral Immunol. 2020 Jul/Aug;33(6):477-488. doi: 10.1089/vim.2019.0178. Epub 2020 Apr 7.
Granulocyte/macrophage colony-stimulating factor (GM-CSF) and macrophage CSF (M-CSF) modulate differentiation and immune functions of macrophages (MΦ). Our aim was to evaluate how different MΦ differentiation conditions influence the MΦ response to virus infection. To address this, we differentiated bone marrow-derived MΦ in either GM-CSF or M-CSF and measured the cytokine responses to two different strains of lymphocytic choriomeningitis virus (LCMV) (clone 13; Cl13 or Armstrong; ARM). GM-CSF MΦ infected with either LCMV-ARM or -Cl13 produced more IL-6 than M-CSF MΦ, whereas M-CSF MΦ generated more IL-10 than GM-CSF MΦ. Interestingly, in M-CSF MΦ, LCMV-ARM induced more IL-10 production than Cl13. However, we could not detect any IL-12p70 or IL-23 after infection from either cell types. We also observed that GM-CSF MΦ was more efficient than M-CSF MΦ in supporting antigen-specific CD8 T cell proliferation. Taken together, our data demonstrate that GM-CSF and M-CSF MΦ differ in how they respond to viral infection by their production of different cytokines, and their support for CD8 T cell proliferation.
粒细胞-巨噬细胞集落刺激因子（GM-CSF）和巨噬细胞集落刺激因子（M-CSF）调节巨噬细胞（MΦ）的分化和免疫功能。我们的目的是评估不同的 MΦ 分化条件如何影响 MΦ 对病毒感染的反应。为了解决这个问题，我们在 GM-CSF 或 M-CSF 中分化骨髓来源的 MΦ，测量对两种不同株淋巴细胞性脉络丛脑膜炎病毒（LCMV）（克隆 13；Cl13 或 Armstrong；ARM）的细胞因子反应。感染 LCMV-ARM 或 -Cl13 的 GM-CSF MΦ 比 M-CSF MΦ 产生更多的 IL-6，而 M-CSF MΦ 比 GM-CSF MΦ 产生更多的 IL-10。有趣的是，在 M-CSF MΦ 中，LCMV-ARM 诱导的 IL-10 产生比 Cl13 更多。然而，我们无法从任何细胞类型检测到感染后的任何 IL-12p70 或 IL-23。我们还观察到 GM-CSF MΦ 比 M-CSF MΦ 更有效地支持抗原特异性 CD8 T 细胞增殖。总之，我们的数据表明 GM-CSF 和 M-CSF MΦ 在对病毒感染的反应方式上存在差异，表现在产生不同的细胞因子和支持 CD8 T 细胞增殖方面。
