Functional Analysis of a Glutamine Biosynthesis Protein from a Psychrotrophic Bacterium, GCJ02.

A putative glutamine synthetase (GS) was detected in a psychrophilic bacterium, GCJ02. For gaining greater insight into its functioning, the gene was cloned and expressed in a heterologous host, . The monomer enzyme with a molecular weight of 53.03 kDa was expressed primarily in cytosolic compartment. The enzyme activity was detected using glutamate and ATP. The optimum conditions of its biosynthesis were observed to be 60 °C and pH value 7.5. Its thermostability was relatively high with a half-life of 50 min at 40 °C. GS activity was enhanced in the presence of metal ions such as Mg and Mn, whereas Fe, Cu and Ca proved inhibitory. The consensus pattern [EXE]-D-KP-[XGXGXH] in the GS lies between residues 132 and 272. The catalytic active sites consisting of EAE and NGSGMH were verified by site-directed mutagenesis. Based on the analysis of the consensus pattern, the GS/glutamate synthase cycle of GCJ02 is expected to contribute to the GS synthesic activity.