Pathak Niti, Verma Neha, Singh Amrita, Bhat K V, Lakhanpaul Suman
1Department of Botany, Ramjas College, University of Delhi, New Delhi, 110007 India.
2Department of Botany, University of Delhi, New Delhi, 110007 India.
Physiol Mol Biol Plants. 2020 Apr;26(4):697-704. doi: 10.1007/s12298-020-00784-4. Epub 2020 Mar 5.
Free radicals, the key mediators of a range of oxidative reactions involved in lipid oxidation are responsible for food quality deterioration leading to several health hazards. Antioxidants synthesized naturally or synthetically are capable of preventing oxidation of lipids and other related compounds. However, natural antioxidants have many benefits over synthetic ones. Sesame seeds contain large amount of natural bioactive components with high antioxidant potential. In the present study, 14 accessions of sesame containing wild species and cultivars were investigated. The antioxidant potential of sesame seed meal extract was evaluated by total phenolic content (TPC) method using Folin-Ciocalteu reagent, linoleic acid peroxidation by Ferric thiocyanate method, and free radical scavenging assay with 2,2-diphenyl-1-picryl hydrazyl radical. showed highest mean values for total polyphenol content with maximum % inhibition of linoleic acid peroxidation on 10th day of course of the reaction span and highest antioxidant scavenging power. subsp. and also showed high total phenol content and radical scavenging capacity. Among the cultivars, Gujarat til 2 showed high TPC and high radical scavenging activity. Higher antioxidant property of species in comparison to sesame cultivars highlights the need to utilize the wild genepool for the improvement of cultigens for enhanced nutraceutical value.
自由基是一系列参与脂质氧化的氧化反应的关键介质，它们会导致食品质量下降并引发多种健康危害。天然合成或人工合成的抗氧化剂能够防止脂质及其他相关化合物的氧化。然而，天然抗氧化剂比合成抗氧化剂有更多益处。芝麻含有大量具有高抗氧化潜力的天然生物活性成分。在本研究中，对14份包含野生种和栽培品种的芝麻材料进行了调查。采用福林-酚试剂法通过总酚含量（TPC）测定芝麻籽粕提取物的抗氧化潜力，采用硫氰酸铁法测定亚油酸过氧化情况，并采用2,2-二苯基-1-苦基肼自由基进行自由基清除试验。在反应过程的第10天，其总多酚含量平均值最高，对亚油酸过氧化的抑制率最高，且抗氧化清除能力最强。 亚种和 也表现出较高的总酚含量和自由基清除能力。在栽培品种中，古吉拉特芝麻2表现出较高的总酚含量和较高的自由基清除活性。与芝麻栽培品种相比， 物种具有更高的抗氧化性能，这突出了利用野生基因库来改良栽培品种以提高营养保健价值的必要性。
