Center for Biosystems Dynamics Research, Riken, Suita, Osaka, Japan.
Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
Contrast Media Mol Imaging. 2020 Mar 12;2020:5425934. doi: 10.1155/2020/5425934. eCollection 2020.
Gas vesicle nanoparticles (GVs) are gas-containing protein assemblies expressed in bacteria and archaea. Recently, GVs have gained considerable attention for biotechnological applications as genetically encodable contrast agents for MRI and ultrasonography. However, at present, the practical use of GVs is hampered by a lack of robust methodology for their induction into mammalian cells. Here, we demonstrate the genetic reconstitution of protein nanoparticles with characteristic bicone structures similar to natural GVs in a human breast cancer cell line KPL-4 and genetic control of their size and shape through expression of reduced sets of humanized gas vesicle genes cloned into Tol2 transposon vectors, referencing the natural gas vesicle gene clusters of the cyanobacteria . We then report the utility of these nanoparticles as multiplexed, sensitive, and genetically encoded contrast agents for hyperpolarized xenon chemical exchange saturation transfer (HyperCEST) MRI.
气室纳米颗粒（GVs）是在细菌和古菌中表达的含气蛋白组装体。最近，GVs 作为可遗传编码的 MRI 和超声对比剂，在生物技术应用中引起了相当大的关注。然而，目前，由于缺乏将 GVs 有效诱导进入哺乳动物细胞的稳健方法，其实际应用受到了阻碍。在这里，我们在人类乳腺癌细胞系 KPL-4 中展示了具有类似天然 GVs 的特征双锥结构的蛋白质纳米颗粒的遗传重建，并通过表达克隆到 Tol2 转座子载体中的简化人类化气室基因，对其大小和形状进行遗传控制，参考了 . 然后，我们报告了这些纳米颗粒作为多相、敏感和遗传编码的对比剂用于超极化氙气化学交换饱和转移（HyperCEST）MRI 的用途。
