Schnurr Matthias, Joseph Roymon, Naugolny-Keisar Alissa, Kaizerman-Kane Dana, Bogdanoff Nils, Schuenke Patrick, Cohen Yoram, Schröder Leif
Molecular Imaging, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125, Berlin, Germany.
School of Chemistry, The Sackler Faculty of Exact Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel.
Chemphyschem. 2019 Jan 21;20(2):246-251. doi: 10.1002/cphc.201800618. Epub 2018 Aug 21.
Macrocyclic host structures for generating transiently bound Xe have been used in various ultra-sensitive NMR and MRI applications for molecular sensing of biochemical analytes. They are based on hyperpolarized nuclei chemical exchange saturation transfer (Hyper-CEST). Here, we tested a set of water-soluble pillar[5]arenes with different counterions in order to compare their potential contrast agent abilities with that of cryptophane-A (CrA), the most widely used host for such purposes. The exchange of Xe with such compounds was found to be sensitive to the type of ions present in solution and can be used for switchable magnetization transfer (MT) contrast that arises from off-resonant pre-saturation. We demonstrate that the adjustable MT magnitude depends on the interplay of saturation parameters and found that the optimum MT contrast surpasses the CrA CEST performance at moderate saturation power. Since modification of such water-soluble pillar[5]arenes is straightforward, these compounds can be considered a promising platform for designing various sensors that may complement the field of Xe HyperCEST-based biosensing MRI.
用于生成瞬态结合氙的大环主体结构已被用于各种超灵敏核磁共振（NMR）和磁共振成像（MRI）应用中，以对生化分析物进行分子传感。它们基于超极化核化学交换饱和转移（Hyper-CEST）。在此，我们测试了一组带有不同抗衡离子的水溶性柱[5]芳烃，以便将它们作为潜在造影剂的能力与隐藻素-A（CrA）进行比较，CrA是此类用途中使用最广泛的主体。发现氙与此类化合物的交换对溶液中存在的离子类型敏感，并且可用于由非共振预饱和产生的可切换磁化转移（MT）对比。我们证明，可调节的MT幅度取决于饱和参数的相互作用，并发现最佳MT对比在中等饱和功率下超过了CrA的CEST性能。由于此类水溶性柱[5]芳烃的修饰很简单，因此这些化合物可被视为一个有前途的平台，用于设计各种传感器，以补充基于氙HyperCEST的生物传感MRI领域。
