Aghaalikhani Nazi, Zamani Mojtaba, Allameh Abdolamir, Mashayekhi Amir, Shadpour Pejman, Mahmoodi Marzieh, Rashtchizadeh Nadereh
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
EXCLI J. 2020 Mar 10;19:351-359. doi: 10.17179/excli2019-1768. eCollection 2020.
The association of haptoglobin (Hp) with various cancers has been reported and also it has been documented that the Hp phenotypes/genotypes have different functional ability. So, we examined phenotypes/genotypes of Hp in newly diagnosed, untreated non-muscle invasive bladder cancer (NMIBC) patients and investigated its prognostic value for risk stratification of the cancer. In eighty NMIBC patients and 80 healthy individuals the Hp genotypes and phenotypes were analyzed using polymerase chain reaction (PCR) and two-dimensional gel electrophoresis (2D-GE), respectively. Besides, the presence of the Hpα1, α2, and β chains in the sera was confirmed by Mass Spectrometry (MS). The frequencies of the and genotypes/phenotypes were respectively higher and lower in healthy subjects compared to the patients. Our results revealed that the genotype/phenotype could increase the risk of NMIBC. There was a positive association between the genotype/phenotype with the T category/grade of cancer (p<0.05). The present study implied a strong association between the Hp phenotypes and genotypes with NMIBC. It was found that the genotype and phenotype could be a risk factor for NMIBC incidence, as well as, progression. This study introduced Hp genotyping as a possible cost-effective and precise method for prognosis of individuals at the risk of NMIBC.
已有报道指出触珠蛋白（Hp）与多种癌症相关，并且也有文献记载Hp的表型/基因型具有不同的功能能力。因此，我们检测了新诊断的、未经治疗的非肌层浸润性膀胱癌（NMIBC）患者的Hp表型/基因型，并研究了其对该癌症风险分层的预后价值。分别采用聚合酶链反应（PCR）和二维凝胶电泳（2D-GE）对80例NMIBC患者和80名健康个体的Hp基因型和表型进行了分析。此外，通过质谱（MS）确认了血清中Hpα1、α2和β链的存在。与患者相比，健康受试者中 和 基因型/表型的频率分别更高和更低。我们的结果显示， 基因型/表型可能会增加NMIBC的风险。 基因型/表型与癌症的T分期/分级之间存在正相关（p<0.05）。本研究表明Hp表型和基因型与NMIBC之间存在密切关联。研究发现， 基因型和表型可能是NMIBC发生以及进展的危险因素。本研究引入了Hp基因分型，作为一种可能具有成本效益且精确的方法，用于对有NMIBC风险的个体进行预后评估。
