Kengne Fotsing Christian Bernard, Pieme Constant Anatole, Biapa Nya Prosper Cabral, Chedjou Jean Paul, Dabou Solange, Nguemeni Carine, Teto Georges, Mbacham Wilfred Fon, Gatsing Donatien
Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science University of Dschang Dschang Cameroon.
Research Unit of Biochemistry of Medicinal Plants, Food Science and Nutrition, Department of Biochemistry, Faculty of Science University of Dschang Dschang Cameroon.
Health Sci Rep. 2022 Jan 21;5(1):e465. doi: 10.1002/hsr2.465. eCollection 2022 Jan.
The haptoglobin (Hp) gene located on chromosome 16q22 exhibits a polymorphism that can impact its capacity to inhibit the deleterious oxidative activity of free hemoglobin. We aimed to determine the influence of Hp polymorphism on oxidative stress, lipid profile, and cardiovascular risk in Cameroonian sickle cell anemia patients (SCA patients).
The Hp genotypes of 102 SCA patients (SS), 60 healthy individuals (AA), and 55 subjects with sickle cell trait (AS) were determined by allele-specific PCR, and the blood parameters were assessed using standard methods.
Hp2-2 genotype was significantly ( < .05) present in SS (54%) than in AS (42%) and AA (38%). Levels of catalase and cell reactive protein were higher, while levels of total antioxidant capacity, triglycerides, low-density lipoprotein cholestetol, blood pressure, Framingham score, and body mass index were lower in the SCA patients. These parameters appeared to be unrelated to the haptoglobin genotypes. SCA patients with Hp1-1 genotype presented a higher oxidative stress index (0.53 ± 0.31) than those with Hp2-1 (0.33 ± 0.18). Lipid profile and cardiovascular risk were not significantly different between various Hp genotypes in SCA patients.
Haptoglobin polymorphism did not affect lipid profile, cardiovascular risk, and oxidative stress status of SCA patients. Nevertheless, SCA patients with Hp1-1 genotype tended to be more prone to oxidative stress than those with Hp2-1.
位于16号染色体q22上的触珠蛋白（Hp）基因存在一种多态性，这种多态性会影响其抑制游离血红蛋白有害氧化活性的能力。我们旨在确定Hp多态性对喀麦隆镰状细胞贫血患者（SCA患者）氧化应激、血脂谱和心血管风险的影响。
采用等位基因特异性PCR法测定102例SCA患者（SS型）、60例健康个体（AA型）和55例镰状细胞性状携带者（AS型）的Hp基因型，并使用标准方法评估血液参数。
SS型患者中Hp2 - 2基因型的比例（54%）显著高于AS型（42%）和AA型（38%）（P < 0.05）。SCA患者的过氧化氢酶和细胞反应蛋白水平较高，而总抗氧化能力、甘油三酯、低密度脂蛋白胆固醇、血压、弗雷明汉评分和体重指数水平较低。这些参数似乎与触珠蛋白基因型无关。Hp1 - 1基因型的SCA患者的氧化应激指数（0.53 ± 0.31）高于Hp2 - 1基因型的患者（0.33 ± 0.18）。SCA患者中不同Hp基因型之间的血脂谱和心血管风险无显著差异。
触珠蛋白多态性不影响SCA患者的血脂谱、心血管风险和氧化应激状态。然而，Hp1 - 1基因型的SCA患者比Hp2 - 1基因型的患者更容易出现氧化应激。
