Zhu Hai-Feng, Liu Zi-Yong, Zhou Xia, Yi Ji-Hong, Lun Zeng-Min, Wang Shu-Ning, Tang Wen-Zhu, Li Fu-Li
School of Biological Engineering, Dalian Polytechnic University, Dalian, China.
Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
Front Microbiol. 2020 Mar 17;11:416. doi: 10.3389/fmicb.2020.00416. eCollection 2020.
Both CO and H can be utilized as energy sources during the autotrophic growth of . In principle, CO is a more energetically and thermodynamically favorable energy source for gas fermentation in comparison to H. Therefore, metabolism may vary during growth under different energy sources. In this study, was fed with CO and/or CO/H at pH 6.0 with a gas pressure of 0.1 MPa. primarily produced acetate in the presence of H as an energy source, but produced alcohols with CO as an energy source under the same fermentation conditions. A key enzyme activity assay, metabolic flux analysis, and comparative transcriptomics were performed for investigating the response mechanism of under different energy sources. A CO dehydrogenase and an aldehyde:ferredoxin oxidoreductase were found to play important roles in CO utilization and alcohol production. Based on these findings, novel metabolic schemes are proposed for growing on CO and/or CO/H. These schemes indicate that more ATP is produced during CO-fermentation than during H-fermentation, leading to increased alcohol production.
在自养生长过程中，一氧化碳（CO）和氢气（H）都可作为能源。原则上，与H相比，CO在气体发酵中是一种在能量和热力学方面更有利的能源。因此，在不同能源下生长时，代谢可能会有所不同。在本研究中，在pH 6.0、气压0.1 MPa的条件下，向（此处原文缺失具体研究对象）供给CO和/或CO/H。在以H作为能源的情况下，（此处原文缺失具体研究对象）主要产生乙酸盐，但在相同发酵条件下以CO作为能源时则产生醇类。为研究（此处原文缺失具体研究对象）在不同能源下的响应机制，进行了关键酶活性测定、代谢通量分析和比较转录组学研究。发现一种CO脱氢酶和一种醛：铁氧还蛋白氧化还原酶在CO利用和醇类生产中起重要作用。基于这些发现，提出了（此处原文缺失具体研究对象）在CO和/或CO/H上生长的新代谢方案。这些方案表明，与H发酵相比，CO发酵过程中产生的三磷酸腺苷（ATP）更多，从而导致醇类产量增加。
