Park Haryung, Cho Donghyun, Huang Eunchong, Seo Ju Yeon, Kim Wan Gi, Todorov Svetoslav Dimitrov, Ji Yosep, Holzapfel Wilhelm Heinrich
Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, South Korea.
Vital Beautie Research Division, Amore Pacific R&D Unit, Gyeonggi-do, South Korea.
Front Microbiol. 2020 Mar 17;11:420. doi: 10.3389/fmicb.2020.00420. eCollection 2020.
Gastric inflammation is an indication of gastric ulcers and possible other underlying gastric malignancies. Epidemiological studies have revealed that several Asian countries, including South Korea, suffer from a high incidence of gastric diseases derived from high levels of stress, alcoholic consumption, pyloric infection and usage of non-steroidal anti-inflammatory drugs (NSAIDs). Clinical treatments of gastric ulcers are generally limited to proton pump inhibitors that neutralize the stomach acid, and the application of antibiotics for eradication, both of which are known to have a negative effect on the gut microbiota. The potential of probiotics for alleviating gastrointestinal diseases such as intestinal bowel syndrome and intestinal bowel disease receives increasing scientific interest. Probiotics may support the amelioration of disease-related symptoms through modulation of the gut microbiota without causing dysbiosis. In this study the potential of APSulloc 331261 (GTB1), isolated from green tea, was investigated for alleviating gastric inflammation in an alcohol induced gastric ulcer murine model (positive control). Treatment with the test strain significantly influenced the expression of pro-inflammatory and anti-inflammatory biomarkers, interleukin 6 (IL6) and interleukin 10 (IL10), of which the former was down- and the latter up-regulated when the alcohol induced mice were treated with the test strain. This positive effect was also indicated by less severe gastric morphological changes and the histological score of the gastric tissues. A significant increase in the abundance of within the GTB1 treated group compared to the positive control group also correlated with a decrease in the ratio of acetate over propionate. The increased levels of propionate in the GTB1 group appear to result from the impact of the test strain on the microbial population and the resulting metabolic activities. Moreover, there was a significant increase in beta-diversity in the group that received GTB1 over that of the alcohol induced control group.
胃炎症是胃溃疡及其他潜在胃部恶性肿瘤的一个指征。流行病学研究表明，包括韩国在内的几个亚洲国家，因压力大、饮酒、幽门感染和使用非甾体抗炎药（NSAIDs），胃部疾病发病率很高。胃溃疡的临床治疗通常局限于使用中和胃酸的质子泵抑制剂以及应用抗生素进行根除治疗，而这两种治疗方法都已知会对肠道微生物群产生负面影响。益生菌缓解诸如肠易激综合征和肠道疾病等胃肠道疾病的潜力受到越来越多的科学关注。益生菌可能通过调节肠道微生物群来支持改善疾病相关症状，而不会导致生态失调。在本研究中，对从绿茶中分离出的APSulloc 331261（GTB1）在酒精诱导的胃溃疡小鼠模型（阳性对照）中缓解胃炎症的潜力进行了研究。用测试菌株进行治疗显著影响了促炎和抗炎生物标志物白细胞介素6（IL6）和白细胞介素10（IL10）的表达，在用测试菌株治疗酒精诱导的小鼠时，前者下调而后者上调。胃形态变化不太严重以及胃组织的组织学评分也表明了这种积极效果。与阳性对照组相比，GTB1治疗组中某物质丰度的显著增加也与乙酸盐与丙酸盐的比例降低相关。GTB1组中丙酸盐水平的升高似乎是由于测试菌株对微生物群落的影响以及由此产生的代谢活动所致。此外，接受GTB1的组与酒精诱导的对照组相比，β多样性显著增加。
